phytochlorin and Carcinoma

phytochlorin has been researched along with Carcinoma* in 3 studies

Other Studies

3 other study(ies) available for phytochlorin and Carcinoma

ArticleYear
Anti-Tumor Efficacy of Photodynamic Therapy of Solid Tumors in Laboratory Animals with Guanidine and Biguanidine Derivatives of Chlorine e6.
    Bulletin of experimental biology and medicine, 2023, Volume: 174, Issue:4

    We evaluated antitumor efficacy of photodynamic therapy of murine Ehrlich carcinoma and rat sarcoma M-1 with new photosensitizers 13

    Topics: Animals; Animals, Laboratory; Carcinoma; Guanidine; Guanidines; Mice; Photochemotherapy; Photosensitizing Agents; Porphyrins

2023
Phototoxic aptamers selectively enter and kill epithelial cancer cells.
    Nucleic acids research, 2009, Volume: 37, Issue:3

    The majority of cancers arise from malignant epithelial cells. We report the design of synthetic oligonucleotides (aptamers) that are only internalized by epithelial cancer cells and can be precisely activated by light to kill such cells. Specifically, phototoxic DNA aptamers were selected to bind to unique short O-glycan-peptide signatures on the surface of breast, colon, lung, ovarian and pancreatic cancer cells. These surface antigens are not present on normal epithelial cells but are internalized and routed through endosomal and Golgi compartments by cancer cells, thus providing a focused mechanism for their intracellular delivery. When modified at their 5' end with the photodynamic therapy agent chlorin e(6) and delivered to epithelial cancer cells, these aptamers exhibited a remarkable enhancement (>500-fold increase) in toxicity upon light activation, compared to the drug alone and were not cytotoxic towards cell types lacking such O-glycan-peptide markers. Our findings suggest that these synthetic oligonucleotide aptamers can serve as delivery vehicles in precisely routing cytotoxic cargoes to and into epithelial cancer cells.

    Topics: Animals; Aptamers, Nucleotide; Carcinoma; Cell Death; Cell Line, Tumor; Chlorophyllides; Cricetinae; Humans; Light; Mucin-1; Photochemotherapy; Photosensitizing Agents; Porphyrins; Protein Isoforms; SELEX Aptamer Technique

2009
Metabolically convertible lipophilic derivatives of pH-sensitive amphipathic photosensitizers.
    Photochemistry and photobiology, 1993, Volume: 58, Issue:6

    We propose the use of acetoxymethyl esters of pH-sensitive amphipathic photosensitizers (PS) for photodynamic therapy (PDT). These compounds may be applicable for PDT involving endocytosis of lipophilic carriers leading to lysosomal uptake of the esterified PS by target cells. Partial and/or total enzymatic de-esterification may result in the extralysosomal distribution of the photoactive agents, possibly culminating in a multisite photochemical response. We report here the synthesis and properties of chlorin e6 triacetoxymethyl ester (CAME) and pheophorbide a acetoxymethyl ester (PAME). Chlorin e6 and pheophorbide a are photocytotoxic chlorins that possess free carboxylate groups and exhibit optimum wavelengths of excitation substantially red shifted relative to hematoporphyrin derivative. Acetoxymethyl esterification of chlorin e6 and pheophorbide a was accomplished with bromomethyl acetate. High-performance liquid chromatography allowed for the purification of PAME, in 87% purity, and CAME, in 63% yield and 94% purity, as well as the detection of the presumed mono- and diesters of chlorin e6 as transient intermediates in the synthesis of CAME. The ultraviolet-visible absorption, fluorescence excitation and emission, NMR and mass spectra of the chlorin e6 triester are consistent with those expected for CAME. The pH-sensitive amphipathicity of pheophorbide a and chlorin e6 but not CAME was demonstrated using a water/1-octanol partition assay. The production of pheophorbide a from PAME and the sequential formation of the di- and monoesters and free chlorin e6 from CAME, by the action of lysosomal esterases obtained from cancer cells, demonstrate the potential of cellular enzymes to convert the lipophilic esters to pH-sensitive amphipathic PS.(ABSTRACT TRUNCATED AT 250 WORDS)

    Topics: Carcinoma; Chlorophyll; Chlorophyllides; Esterases; Esterification; Esters; Humans; Hydrogen-Ion Concentration; Lysosomes; Photosensitizing Agents; Porphyrins; Solubility; Spectrometry, Fluorescence; Spectrophotometry; Urinary Bladder Neoplasms

1993