phytochlorin and Carcinoma--Lewis-Lung

Nanotechnology has emerged as a promising solution to permanent elimination of cancer. However, nanoparticles themselves lack specificity to tumors. Due to enhanced migration to tumors, mesenchymal stem cells (MSCs) were suggested as cell-mediated delivery vehicles of nanoparticles. In this study, we have constructed a complex composed of photoluminescent quantum dots (QDs) and a photosensitizer chlorin e6 (Ce6) to obtain multifunctional nanoparticles, combining cancer diagnostic and therapeutic properties. QDs serve as energy donors-excited QDs transfer energy to the attached Ce6

Topics: Animals; Antineoplastic Agents; Cadmium Compounds; Carcinoma, Lewis Lung; Cell Line, Tumor; Chlorophyllides; Female; Humans; Light; Mesenchymal Stem Cells; Mice, Inbred C57BL; Multifunctional Nanoparticles; Photochemotherapy; Photosensitizing Agents; Precision Medicine; Quantum Dots; Selenium Compounds; Singlet Oxygen; Sulfides; Zinc Compounds

Photodynamic therapy (PDT) is an emerging anti-tumor strategy.Photosensitizer chlorin e6 (Ce6) can induce photodynamic effect to selectively damage lung cancer cells.In order to further improve its tumor targeting ability, macrophages can be applied as carrier to deliver Ce6 to lung cancer.Tumor associated macrophages (TAM) are important immunocytes in lung cancer immune microenvironment. TAM play crucial role in tumor promotion due to the Immunosuppressive property, reprogramming phenotype of TAM therefore has become a promising strategy.Based on this, in the present study, we suppose that TAM can be used as carrier to deliver Ce6 to lung cancer and be reprogrammed to M1 phenotype by photodynamic action to mediate anti-lung cancer efficacy.The results showed TAM could load with Ce6 and keep viability in the absence of near infrared irradiation (NIR).Moreover, Its viability decreased little within 10 h after NIR.Ce6-loaded TAM could deliver Ce6 to lung cancer cells and retain some drugs in TAM per se.After NIR, phagocytosis of macrophages was enhanced. The expressions of GBP5, iNOS and MHC-II was up-regulated, which indicated TAM were polarized to M1 phenotype.Finally, the study also found the reprogrammed macrophages could inhibit the proliferation and promote the apoptosis of lung cancer cells.These results suggested that macrophages could deliver Ce6 to lung cancer and exhibit anti-lung cancer effect through photodynamic reprogramming.This study provides a novel approach for combining photodynamic action with anti-tumor immunotherapy.

Topics: Animals; Apoptosis; Carcinoma, Lewis Lung; Cell Line, Tumor; Cell Proliferation; Chlorophyllides; Coculture Techniques; Immunotherapy; Lung Neoplasms; Mice; Phagocytosis; Phenotype; Photochemotherapy; Radiation-Sensitizing Agents; RAW 264.7 Cells; Tumor Microenvironment; Tumor-Associated Macrophages