phytochlorin and Body-Weight

Sonodynamic Therapy (SDT) has good targeting and non-invasive advantages in solid cancers, but its antitumor effect is not sufficient to replace traditional treatments. Some studies that combined SDT with chemotherapy or nanoparticles have managed to enhance its efficiency and overcome the side effects of chemotherapy.. In this study, we synthesized and characterized mesoporous silica nanoparticles (MSN-DOX-Ce6) loaded with doxorubicin (DOX) and sonosensitizer, chlorin e6 (Ce6). Then, we conducted in vitro and in vivo experiments to explore the antitumor effect of MSN-DOX-Ce6 under ultrasound (US) treatment.. The characterization tests showed that the nanoparticles are uniformly sized spheres with mesoporous structure, resulting in a high drug-loading efficiency. In the in vitro experiments, MSN-DOX-Ce6 could effectively inhibit cell proliferation under US but not more than other treatment groups. However, the in vivo studies showed that MSN-DOX-Ce6+US has better antitumor effect than DOX+Ce6+US or DOX alone on xenograft tumor-bearing mice.. In summary, MSNs showed a great potential for DOX and Ce6 delivery. We concluded that under US, MSN-DOX-Ce6 nanocomposites increase the antitumor effect of DOX and SDT and thereby are a potential treatment for solid tumors.

Topics: Animals; Antineoplastic Agents; Body Weight; Cell Line, Tumor; Chlorophyllides; Doxorubicin; Drug Liberation; Fluorescence; Humans; Mice; Nanoparticles; Porosity; Porphyrins; Silicon Dioxide; Triple Negative Breast Neoplasms; Tumor Burden; Ultrasonography

Topics: Animals; Body Weight; Cell Survival; Chlorophyllides; Diagnostic Imaging; Disulfides; HeLa Cells; Humans; Infrared Rays; Magnetic Resonance Imaging; Mice, Inbred BALB C; Molybdenum; Nanoparticles; Organ Specificity; Phototherapy; Porphyrins; Silicon Dioxide; Tomography, X-Ray Computed; Tumor Burden; X-Ray Diffraction

Derivatives of porphyrin, specifically Fe-chlorin e6-Na (FeCNa), mimic superoxide dismutase (SOD). This SOD activity was determined by decrease in electron spin resonance (ESR) signals and increase in hydrogen peroxide (H2O2) as the intermediate of O2-. by the coloration using 4-aminoantipyrin. Chlorin e6-Na used for cancer photodynamic therapy (PDT)(1) does not show SOD mimicking activity. The specific activity of FeCNa, comparing with bovine RBC-SOD, was 1/7.5 as determined by ESR analysis. The iron element of Fe-chlorin e6-Na, being tightly encased in the molecule, did not participate in the Fenton reaction. The SOD mimetic activity of FeCNa was stable against physico-chemical treatment such as pH shock, heat and digestion by pronase. For cancer bearing rats with oxidative stress (OS), immediate relief of OS was possible by a single intraperitoneal injection of FeCNa and relief continued for 24 hours. The subsequent administration of FeCNa suppressed cancer growth in vivo.

Topics: Animals; Body Weight; Chlorophyllides; Electron Spin Resonance Spectroscopy; Fibrosarcoma; Hydrogen Peroxide; Injections, Intraperitoneal; Neoplasms, Experimental; Oxidative Stress; Oxygen; Porphyrins; Radiation-Sensitizing Agents; Rats; Rats, Wistar; Superoxide Dismutase