phycocyanobilin and Chromosome-Deletion

Recombinant pea type I phytochrome apoprotein expressed in yeast is shown to assemble in vitro with phycocyanobilin to produce a photoreversible phytochrome-like adduct. As an initial investigation of the amino acid sequence requirements for chromophore incorporation, three phyA gene product deletion mutants were produced in yeast. Truncation of the N-terminal tail to residue 46 demonstrates that this region is not critical to bilin attachment, but a deletion mutant lacking 222 amino acids from the N terminus failed to yield holophytochrome in vitro, under the same conditions. A mutant comprising a deletion of the C terminus to residue 548 showed bilin incorporation and red/far-red photoreversibility, indicating that bilin-apophytochrome assembly still occurred even when the entire C-terminal domain was truncated.

Topics: Apoproteins; Base Sequence; Chromosome Deletion; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fabaceae; Molecular Sequence Data; Oligodeoxyribonucleotides; Phycobilins; Phycocyanin; Phytochrome; Plants, Medicinal; Pyrroles; Recombinant Fusion Proteins; Restriction Mapping; Saccharomyces cerevisiae; Spectrophotometry; Tetrapyrroles