phosphorus-radioisotopes and Uveitis

For determination of the presence and activity of inflammation in the vascular coat of the eye as well as of the stopping of the disease, the authors propose to use radionuclide 32-P in a form of fractional doses in contradistinction to a single administration of a conventional dose. A common dose of the preparation is divided into four equal parts. At first, 1/4 of the dose is administered. One hour later, comparative external radiometry of the patient and the healthy eye is made. If there is seen no clear difference in intensity of accumulation, the second dose is administered. One hour later, repeated examination is made. After receiving clear data, further administration of the preparation is stopped. Administration of fractional doses suggests a decrease of ray load on the patient's organism, economy of the preparation without lowering the authenticity of examination.

Topics: Humans; Male; Methods; Middle Aged; Phosphorus Radioisotopes; Radionuclide Imaging; Time Factors; Uveitis

Topics: Ear, External; Eye; Humans; Methods; Phosphorus Radioisotopes; Radionuclide Imaging; Uveitis

Topics: Adult; Female; Humans; Male; Middle Aged; Phosphorus Radioisotopes; Prognosis; Uveitis

Topics: Animals; Disease Models, Animal; Eye; Hypersensitivity; Phosphorus Radioisotopes; Rabbits; Time Factors; Uveitis

A single systemic injection of endotoxin (lipopolysaccharide or LPS) reproducibly induces a cellular infiltrate in the uveal tract of the rat eye within 24 hr. Other organs are not comparably sensitive to systemic endotoxin. One hypothesis to explain this unique sensitivity is that endotoxin is preferentially bound by ocular tissue. We tested this hypothesis by studying the distribution in the rat of intravenously injected endotoxin that had been radiolabeled with Tc-99m or P-32. With either radionuclide the concentration of endotoxin per gram of tissue at a variety of times after injection ranging from 5 min to 3 hr and 45 min, was markedly less in the eye than in liver, kidney, or spleen. A study with radiolabeled albumin indicated that these differences could not be ascribed solely to the organ's blood volume. They demonstrate, therefore, that the eye does not preferentially bind endotoxin, and they are compatible with the hypothesis that endotoxin's ocular effects are indirectly mediated.

Topics: Animals; Endotoxins; Injections, Intravenous; Kidney; Lipopolysaccharides; Liver; Male; Phosphorus Radioisotopes; Rats; Rats, Inbred Strains; Spleen; Technetium; Time Factors; Uvea; Uveitis