phosphorus-radioisotopes and Thalassemia

Slot blot analysis was used to differentiate between the genotypes of homozygous alpha-thalassaemia and those of haemoglobin-H disease and alpha-thalassaemia trait. The method is simple and rapid because restriction enzyme digestion and gel electrophoresis are not needed. Because of the great sensitivity of the test, as little as 0.5 micrograms of DNA can be studied with an 35S-labelled probe which has a longer isotopic half life than the 32P probes normally used for gene mapping. This assay should facilitate prenatal diagnosis in countries where pregnancies at risk for alpha-thalassaemia are common.

Topics: Amnion; DNA; Edema; Female; Fetal Diseases; Globins; Homozygote; Humans; Infant, Newborn; Nucleic Acid Hybridization; Phosphorus Radioisotopes; Pregnancy; Prenatal Diagnosis; Sulfur Radioisotopes; Thalassemia

Topics: Amniotic Fluid; Autoradiography; DNA; DNA Restriction Enzymes; Electrophoresis, Agar Gel; Female; Genes; Globins; Hemoglobins, Abnormal; Humans; Nucleic Acid Hybridization; Phosphorus Radioisotopes; Pregnancy; Thalassemia

Topics: Chromosome Aberrations; Chromosome Mapping; DNA; Edema; Female; Fetal Diseases; Genes; Genetic Linkage; Globins; Hemoglobin H; Hemoglobins, Abnormal; Humans; Hydroxyapatites; Phosphorus Radioisotopes; Pregnancy; RNA, Messenger; Thalassemia; Tritium

Topics: Anemia, Hemolytic; Animals; Avian Myeloblastosis Virus; Bone Marrow; Bone Marrow Cells; DNA; Fetal Hemoglobin; Globins; Hemoglobins; Humans; Nucleic Acid Hybridization; Peptide Fragments; Phosphorus Radioisotopes; Rabbits; Reticulocytes; Ribosomes; RNA-Directed DNA Polymerase; RNA, Messenger; Thalassemia; Threonine; Tritium

Topics: Base Sequence; Cell-Free System; China; DNA; Genes; Hemoglobins, Abnormal; Homozygote; Humans; Infant, Newborn; Nucleic Acid Hybridization; Phosphorus Radioisotopes; Protein Biosynthesis; RNA, Messenger; Thalassemia; Transcription, Genetic; Tritium

Topics: Adult; Aged; Anemia, Hemolytic; Anemia, Hypochromic; Anemia, Macrocytic; Bone Marrow; Cell Survival; Erythrocytes; Erythropoiesis; Female; Hematologic Diseases; Hemoglobins; Humans; Iron; Iron Radioisotopes; Isoflurophate; Leukemia, Lymphoid; Liver Cirrhosis; Male; Methods; Middle Aged; Phosphorus Radioisotopes; Polycythemia; Spherocytosis, Hereditary; Thalassemia

Topics: Adenosine Triphosphate; Anemia, Hemolytic; Anemia, Sickle Cell; Autoradiography; Avian Myeloblastosis Virus; Cell-Free System; Centrifugation, Density Gradient; Chromatography, Affinity; Chromatography, DEAE-Cellulose; DNA; DNA Nucleotidyltransferases; Electrophoresis, Disc; Escherichia coli; Globins; Hemoglobin H; Hemoglobinopathies; Hemolysis; Humans; Nucleic Acid Hybridization; Oligonucleotides; Pancreas; Phosphorus Radioisotopes; Poly U; Reticulocytes; Ribonucleases; RNA, Messenger; Thalassemia; Ultracentrifugation