phosphorus-radioisotopes and Multiple-Sclerosis

phosphorus-radioisotopes has been researched along with Multiple-Sclerosis* in 2 studies

Other Studies

2 other study(ies) available for phosphorus-radioisotopes and Multiple-Sclerosis

Article	Year
Radioactive 33-P probes in hybridization to glass cDNA microarrays using neural tissues. Journal of neuroscience methods, 2001, Mar-30, Volume: 106, Issue:1 cDNA microarrays are becoming widespread tools in the study of complex gene expression patterns with applications using cells lines, animal model systems, and human disease. Glass cDNA microarrays using fluorescent labeled cDNA probes require a large amount of input RNA usually not available in many neuroscience applications. Here we demonstrate a technique for the use of 33-P labeled cDNA probes in hybridizations to the same glass cDNA arrays used for fluorescent applications. This approach allows the use of low quantities of RNA, common phosphoimaging scanners, data acquisition software, and standard DNA and RNA labeling protocols, while being consistent and interchangeable with glass-based cDNA array technology. Topics: DNA Probes; DNA, Complementary; Gene Expression; Humans; Multiple Sclerosis; Oligonucleotide Array Sequence Analysis; Phosphorus Radioisotopes; RNA	2001
Proteins from human cerebrospinal fluid: binding with nucleic acids. Science (New York, N.Y.), 1973, Oct-19, Volume: 182, Issue:4109 Cerebrospinal fluid (CSF) contains two groups of proteins that bind tightly to DNA and to polyriboguanylic acid, respectively. In certain diseases the amounts of a given nucleic acid bound by a constant volume of CSF may increase, while in others the amount of such proteins may be reduced. Binding of polyriboguanylic acid increased in CSF samples from patients with brain tumors, stroke, multiple sclerosis, and communicating hydrocephalus, but it significantly decreased in CSF samples from patients with obstructive hydrocephalus. These increases may or may not be proportional to the rise in total CSF proteins characteristic for these diseases. Elevated binding of DNA was observed in samples from patients with hydrocephalus, epilepsy, and cortical atrophy. The technique described may be applicable to the diagnosis of a variety of diseases of the central nervous system. Topics: Alcoholism; Astrocytoma; Brain Diseases; Brain Injuries; Brain Neoplasms; Carcinoma; Cerebrospinal Fluid Proteins; Cerebrovascular Disorders; Child, Preschool; DNA; Epilepsy; Female; Guanine Nucleotides; Headache; Humans; Hydrocephalus; Meningioma; Middle Aged; Multiple Sclerosis; Neurilemmoma; Phosphorus Radioisotopes; Polynucleotides; Protein Binding; Schizophrenia; Tritium	1973

Article

Year

Radioactive 33-P probes in hybridization to glass cDNA microarrays using neural tissues.

Journal of neuroscience methods, 2001, Mar-30, Volume: 106, Issue:1

cDNA microarrays are becoming widespread tools in the study of complex gene expression patterns with applications using cells lines, animal model systems, and human disease. Glass cDNA microarrays using fluorescent labeled cDNA probes require a large amount of input RNA usually not available in many neuroscience applications. Here we demonstrate a technique for the use of 33-P labeled cDNA probes in hybridizations to the same glass cDNA arrays used for fluorescent applications. This approach allows the use of low quantities of RNA, common phosphoimaging scanners, data acquisition software, and standard DNA and RNA labeling protocols, while being consistent and interchangeable with glass-based cDNA array technology.

Topics: DNA Probes; DNA, Complementary; Gene Expression; Humans; Multiple Sclerosis; Oligonucleotide Array Sequence Analysis; Phosphorus Radioisotopes; RNA

2001

Proteins from human cerebrospinal fluid: binding with nucleic acids.

Science (New York, N.Y.), 1973, Oct-19, Volume: 182, Issue:4109

Cerebrospinal fluid (CSF) contains two groups of proteins that bind tightly to DNA and to polyriboguanylic acid, respectively. In certain diseases the amounts of a given nucleic acid bound by a constant volume of CSF may increase, while in others the amount of such proteins may be reduced. Binding of polyriboguanylic acid increased in CSF samples from patients with brain tumors, stroke, multiple sclerosis, and communicating hydrocephalus, but it significantly decreased in CSF samples from patients with obstructive hydrocephalus. These increases may or may not be proportional to the rise in total CSF proteins characteristic for these diseases. Elevated binding of DNA was observed in samples from patients with hydrocephalus, epilepsy, and cortical atrophy. The technique described may be applicable to the diagnosis of a variety of diseases of the central nervous system.

Topics: Alcoholism; Astrocytoma; Brain Diseases; Brain Injuries; Brain Neoplasms; Carcinoma; Cerebrospinal Fluid Proteins; Cerebrovascular Disorders; Child, Preschool; DNA; Epilepsy; Female; Guanine Nucleotides; Headache; Humans; Hydrocephalus; Meningioma; Middle Aged; Multiple Sclerosis; Neurilemmoma; Phosphorus Radioisotopes; Polynucleotides; Protein Binding; Schizophrenia; Tritium

1973