phosphorus-radioisotopes and Infections

Recent developments in recombinant DNA technology have made possible the production of gene probes consisting of cloned gene segments, cloned segments of DNA linked to genes, and synthetic gene fragments. Several methods have been developed by which these probes may be used for the diagnosis of human disease. This technology has been outstandingly successful for prenatal diagnosis and carrier detection in many genetic diseases. These methods have also been successfully applied to the analysis of human malignancies, by providing for the determination of cell lineage and clonality in lymphoid neoplasms. Finally, these methods have shown potential for rapid and sensitive diagnosis of some infectious diseases.

Topics: B-Lymphocytes; Bacterial Infections; Biotin; Cloning, Molecular; Deoxyribonucleotides; DNA; DNA Restriction Enzymes; DNA, Recombinant; Female; Genetic Carrier Screening; Genetic Diseases, Inborn; Genotype; Hemophilia A; Humans; Immunoglobulins; Infections; Leukemia, Lymphoid; Leukocytes; Male; Muscular Dystrophies; Nucleic Acid Hybridization; Phenylketonurias; Phosphorus Radioisotopes; Polymorphism, Genetic; Pregnancy; Prenatal Diagnosis; Receptors, Antigen, T-Cell; RNA; T-Lymphocytes; Virus Diseases

The purpose of this study was to investigate possible alterations induced by sepsis and endotoxicosis in the late phase of Ca2+-dependent signaling in rat liver. Hepatocytes isolated from septic or chronically endotoxin (ET)-treated rats were labeled with [32P]H3PO4 and stimulated with various agents. Proteins were resolved by one-dimensional polyacrylamide gel electrophoresis and autoradiographed. Vasopressin (VP)- and phenylephrine (PE)-induced responses were attenuated in both septic and ET-treated rats for cytosolic and membrane proteins compared with their respective controls. Glucagon and 12-O-myristate phorbol-13-acetate (TPA) affected only the phosphorylation of membrane proteins. Glucagon-induced changes in the phosphorylation of membrane proteins were affected by both sepsis and endotoxicosis, whereas TPA-stimulated phosphorylation was lowered only in endotoxicosis. Response to the Ca2+ ionophore A23187 was depressed in septic rats for cytosolic proteins. The phosphorylation of two cytosolic proteins, i.e., 93 and 61 kDa (previously identified as glycogen phosphorylase and pyruvate kinase, respectively), in response to VP, PE, and A23187 was severely impaired by endotoxicosis and sepsis. TPA did not affect the phosphorylation state of these two proteins. The results show that sepsis and endotoxicosis produce perturbations of the phosphorylation step in Ca2+ transmembrane signaling. Such changes can explain alterations of glycogenolysis and gluconeogenesis associated with sepsis and endotoxicosis.

Topics: Adenosine Triphosphate; Animals; Cell Separation; Digitonin; Endotoxins; Infections; Liver; Male; Phosphorus Radioisotopes; Phosphorylation; Proteins; Rats; Rats, Inbred Strains; Stimulation, Chemical

Topics: Bone and Bones; Bone Diseases; Calcium Radioisotopes; Diagnosis, Differential; Humans; Infections; Necrosis; Phosphorus Radioisotopes; Prostheses and Implants; Radioisotopes; Radionuclide Imaging; Strontium Isotopes; Strontium Radioisotopes

Topics: Aged; B-Lymphocytes; Chlorambucil; Chronic Disease; Female; Humans; Infections; Leukemia, Lymphoid; Male; Middle Aged; Phosphorus Radioisotopes; Prednisone; Prognosis

Topics: Adolescent; Adult; Aged; Child; Female; Half-Life; Humans; Infections; Inflammation; Isoflurophate; Kinetics; Leukocyte Count; Leukocytes; Male; Middle Aged; Monocytes; Neutrophils; Phosphorus Radioisotopes; Prednisone; Skin Window Technique; Time Factors