phosphorus-radioisotopes has been researched along with Dermatitis--Atopic* in 3 studies
3 other study(ies) available for phosphorus-radioisotopes and Dermatitis--Atopic
| Article | Year |
|---|---|
Incorporation of radioactive phosphate from adenosine triphosphate into phosphatidylinositol-4-phosphate in human peripheral blood hypodense eosinophils is less than in the normodense eosinophils.
Peripheral blood eosinophils from patients with atopic dermatitis and normal healthy controls were isolated on a Percoll gradient and incubated with [gamma 32P]ATP in the presence of Mg2+. After stopping the reaction, lipid extraction was performed with acidic medium and phospholipids were separated by thin-layer chromatography on 1% (w/v) oxalic acid-impregnated silica gel plates. In the autoradiographs considerable amounts of the radioactivity were found to be incorporated into phosphatidylinositol-4-phosphate. 32P incorporation into phosphatidylinositol-4-phosphate in hypodense eosinophils from atopic dermatitis patients was less than in normodense eosinophils from the same patients and controls. Topics: Adenosine Triphosphate; Adolescent; Adult; Autoradiography; Cell Count; Chromatography, Thin Layer; Dermatitis, Atopic; Eosinophils; Female; Humans; Male; Phosphates; Phosphatidylinositol Phosphates; Phosphorus Radioisotopes | 1994 |
Human peripheral blood hypodense eosinophil proteins are more labeled with 32P than the normodense eosinophil proteins.
Peripheral blood eosinophils from patients with atopic dermatitis and normal healthy controls were isolated on a Percoll gradient and were incubated with [32P]orthophosphoric acid. After stopping the reaction, SDS/PAGE was performed and autoradiographs were prepared to determine the incorporation of 32P into proteins. Eosinophils developed at least 14 protein bands below 66.2 kDa by SDS/PAGE and the differences of the protein staining patterns between hypodense and normodense eosinophils were present. In the autoradiographs five distinct radioactive bands were observed below 31 kDa. 32P incorporation into the bands of hypodense eosinophils was stronger than that of normodense eosinophils, suggesting possible involvement of protein phosphorylation in the activation process of human eosinophils. Topics: Adult; Autoradiography; Blood Proteins; Centrifugation, Density Gradient; Dermatitis, Atopic; Electrophoresis, Polyacrylamide Gel; Eosinophils; Female; Humans; Male; Phosphorus Radioisotopes; Phosphorylation | 1992 |
[Hypodense eosinophils are much labeled with 32P than normodense eosinophils].
Peripheral blood eosinophils from the patients with atopic dermatitis were isolated on a Percoll gradient and incubated with H3(32)PO4. After stopping the reaction, SDS/PAG electrophoresis was performed and autoradiographs were prepared to determine the incorporation of 32P into proteins. Eosinophils developed at least 14 protein bands below 66.2 K by SDS/PAG electrophoresis and the differences of the staining patterns between hypodense and normodense eosinophils were observed. In the autoradiographs 5 distinct radioactive bands were observed below 31 K. 32P incorporation into the bands of hypodense eosinophils were stronger than that of normodense eosinophils, suggesting possible involvement of protein phosphorylation in the activation process of eosinophils. Topics: Adult; Dermatitis, Atopic; Eosinophils; Female; Humans; Male; Phosphorus Radioisotopes; Phosphorylation; Specific Gravity | 1991 |