phosphoramidite has been researched along with Hepatitis-D* in 1 studies
1 other study(ies) available for phosphoramidite and Hepatitis-D
Article | Year |
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Evidence That Nucleophile Deprotonation Exceeds Bond Formation in the HDV Ribozyme Transition State.
Steric constraints imposed by the active sites of protein and RNA enzymes pose major challenges to the investigation of structure-function relationships within these systems. As a strategy to circumvent such constraints in the HDV ribozyme, we have synthesized phosphoramidites from propanediol derivatives and incorporated them at the 5'-termini of RNA and DNA oligonucleotides to generate a series of novel substrates with nucleophiles perturbed electronically through geminal fluorination. In nonenzymatic, hydroxide-catalyzed intramolecular transphosphorylation of the DNA substrates, pH-rate profiles revealed that fluorine substitution reduces the maximal rate and the kinetic p K Topics: DNA; Hepatitis D; Hepatitis Delta Virus; Humans; Nucleic Acid Conformation; Oligonucleotides; Organophosphorus Compounds; Protons; RNA, Catalytic; RNA, Viral; Substrate Specificity | 2018 |