phosphocreatine and Swine-Diseases

31P nuclear magnetic resonance spectroscopy was performed on 19 to 55 kg weighing pigs of different MHS genotypes to study the changes of phosphorus components (inorganic phosphate --Pi, phosphocreatine--PCr and adenosine triphosphate--ATP) of muscle metabolism as well as intramuscular pH under application of halothane. Aim of the present study was to observe the changes in energy metabolism and to perform a comparison with also measured blood parameters. Both, NN and Nn pigs did not show any changes during halothane exposure in phosphorus spectra, but in all animals a partially metabolically compensated respiratoric acidosis was found. In all MHS positive pigs a rapid fall of PCr and a corresponding raise of Pi levels in muscle was observed.

Topics: Adenosine Triphosphate; Animals; Disease Susceptibility; Energy Metabolism; Genotype; Magnetic Resonance Spectroscopy; Muscle, Skeletal; Phosphates; Phosphocreatine; Stress, Physiological; Swine; Swine Diseases

Energy metabolism of skeletal muscle tissue of pigs growing from approximately 12 to 18 kg (12 homozygous halothane negative, HH; 16 heterozygotes, Hh; 17 homozygous halothane susceptible, hh) was measured in vivo using 31P nuclear magnetic resonance (NMR) spectroscopy. Data for intracellular pH, phosphocreatine (PCr), phosphomonoesters (PME), and ATP were analyzed by canonical discriminant analysis, an artificial neural network approach, and analysis of variance. Within the hh pigs, two subpopulations could be distinguished before the application of halothane treatment. Some of the hh pigs had a high PME concentration in the biceps femoris muscle (hh(pme+)), whereas others had a low concentration (hh(pme-)) (2.18 +/- .12 for hh(pme+) vs 1.68 +/- .12 mM for hh(pme-), P < .004). The hh(pme+) pigs were statistically different from HH pigs for pH (P < .03), PME (P < .004), and PCr (P < .008) before halothane treatment. The hh(pme-) pigs were not different from the Hh and HH pigs with respect to PME when measured before halothane treatment (P > .05). However, intracellular pH (P < .03) and PCr (P < .008) of the hh(pme-) pigs were different from those of HH pigs (7.15 vs 7.19 for pH and 38.7 vs 35.1 for PCr, respectively). When combining intracellular pH, PME, and PCr within a canonical discriminant analysis, all were measured before halothane treatment, Hh pigs were found to be different from HH pigs (Mahalanobis distance different from zero, P < .02). In a second experiment, growth rate, depth of longissimus muscle, and maximal binding capacity of nuclear T3-receptors of skeletal muscle tissue were different (P < .05, P < .002, and P < .02, respectively) among pigs selected from the same genetic lines. Of the variability in depth of the longissimus muscle, 22% was explained by variability in maximal binding capacity of nuclear T3-receptors. These results, if confirmed with a large number of pigs, might open new possibilities for selection procedures for leanness because, with respect to halothane susceptibility, a shift between genotypic and phenotypic variability was observed.

Topics: Adenosine Triphosphate; Animals; Cell Nucleus; Energy Metabolism; Female; Genetic Variation; Genotype; Homozygote; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Male; Malignant Hyperthermia; Muscle, Skeletal; Phenotype; Phosphocreatine; Random Allocation; Receptors, Thyroid Hormone; Swine; Swine Diseases

Muscle metabolism was studied in pigs of different halothane genotypes by taking blood and muscle biopsy samples during a 45-min preslaughter period of anesthesia. Dantrolene was administered to half the pigs of each genotype to investigate whether possible differences in muscle metabolism could be explained by differences in resting myoplasmic calcium concentrations. Dantrolene influenced muscle metabolism of all halothane genotypes to the same extent, leading to higher (P < .05) glycogen and creatine phosphate concentrations and lower (P < .05) lactate and creatine concentrations. Dantrolene could not reduce the small but significant (P < .05) differences observed in resting muscle metabolism between the genotypes, and halothane-positive pigs had lower (P < .05) glycogen and creatine phosphate contents. Postmortem muscle metabolism showed differences (P < .05) between the three halothane genotypes, with the highest rate of glycolysis in halothane-positive pigs. Dantrolene resulted in a significantly slower (P < .05) glycogen and creatine-P breakdown, which led to a less rapid (P < .05) accumulation of lactate and creatine in both muscles. Meat quality characteristics differed between the halothane genotypes but no PSE meat was detected. Dantrolene administration resulted in an elevation (P < .05) of the pH in the longissimus lumborum and in lower (P < .05) rigor values of the semimembranosus, both measured at 45 min after slaughter. Water-holding capacity was higher (P < .05) and Hunter L*-values lower (P < .05) is dantrolene-treated pigs than in the control animals.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Anesthesia; Animals; Biopsy, Needle; Calcium; Creatine; Dantrolene; Genotype; Glycogen; Halothane; Hydrogen-Ion Concentration; Lactates; Male; Malignant Hyperthermia; Meat; Muscle, Skeletal; Phosphocreatine; Stress, Physiological; Swine; Swine Diseases

In vivo muscle 31P nuclear magnetic resonance spectroscopy was performed on 12 homozygous halothane-nonsensitive female pigs and 13 female pigs heterozygous with respect to the halothane gene. Fifteen female pigs of a third line, consisting of heterozygotes and halothane-nonsensitive homozygotes, were also available. Body weight ranged from 12 to 18 kg. Mean decrease in phosphocreatine concentration in the biceps femoris of anesthetized pigs was significantly lower for heterozygous vs homozygous pigs (3.46% vs 5.94%, P less than 0.01) after 40 minutes of halothane exposure (3%; oxygen flow, 3 L/min). Also, a statistically significant difference, with respect to the initial (7.21 vs 7.11, P less than 0.008) and end muscle pH values (7.18 vs 7.06, P less than 0.0002), was observed for homozygous vs heterozygous pigs. By means of canonical discriminant analysis, it was possible to distinguish nonsensitive homozygotes from heterozygotes (P less than 0.0001). When applying this classification method to pigs of the same strain, 2 populations (nonsensitive homozygotes, heterozygotes) emerged, with a proportion of pigs corresponding to the expected value on the basis of breeding records. In contrast to the phenotypic expression of muscular rigidity related to the malignant hyperthermia syndrome, the expression of metabolic variables (phosphocreatine, pH) was shown to be dominant.

Topics: Animals; Breeding; Female; Genotype; Halothane; Heterozygote; Homozygote; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Malignant Hyperthermia; Muscles; Phosphocreatine; Swine; Swine Diseases

In vivo muscle 31P nuclear magnetic resonance spectroscopy was performed on 10 female pigs originating from a homozygous halothane-sensitive line and on 10 female pigs from a homozygous halothane-nonsensitive line. The mean concentration of phosphocreatine in the biceps femoris muscle of the anesthetized pigs decreased to 86% of the initial value after 11 minutes of halothane exposure (3%, oxygen flow 3 L/min). After the next 5.6 minutes, phosphocreatine concentration reached a minimal value of 52%, followed by a mean recovery to 76% of the initial value during the ensuing 11 minutes. Response was not observed in anesthetized homozygous halothane-nonsensitive pigs. Thus, a decrease to 86% of the initial value of phosphocreatine was 100% predictive for homozygous halothane-sensitive pigs with body weight ranging from 10 to 18 kg.

Topics: Adenosine Triphosphate; Animals; Female; Halothane; Magnetic Resonance Spectroscopy; Malignant Hyperthermia; Muscles; Phosphocreatine; Swine; Swine Diseases

A technique of obtaining muscle biopsies with a liquid nitrogen cryoprobe was used to study intramuscular metabolites in MH-susceptible and unsusceptible pigs. There was no significant difference in muscle metabolite values obtained from susceptible and unsusceptible pigs in the resting state. During MH the changes in metabolites were a result of rapid glycogenolysis and no abnormality of glycolytic control was observed.

Topics: Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphate; Animals; Creatine; Glycogen; Lactates; Malignant Hyperthermia; Muscles; Phosphates; Phosphocreatine; Swine; Swine Diseases

Topics: Adenosine Triphosphate; Anesthesia, Intravenous; Animals; Glucosephosphates; Halothane; Lactates; Malignant Hyperthermia; Muscles; Pentobarbital; Phosphocreatine; Swine; Swine Diseases; Syndrome

Topics: Adenosine Triphosphate; Animals; Glucosephosphates; Lactates; Male; Myocardium; Phosphocreatine; Stress, Physiological; Swine; Swine Diseases

Topics: Acid-Base Equilibrium; Adenosine Triphosphate; Animals; Body Temperature; Carbon Dioxide; Fever; Glucosephosphates; Hot Temperature; Hydrogen-Ion Concentration; Lactates; Muscles; Oxygen; Phosphocreatine; Physical Exertion; Rectum; Stress, Physiological; Swine; Swine Diseases

Topics: Acetylcholinesterase; Adenosine Triphosphate; Animals; Electromyography; Lactates; Membrane Potentials; Muscles; Phosphocreatine; Stress, Physiological; Swine; Swine Diseases