phosphocreatine and Peritonitis

The effect of citoflavin and neoton preparations on the migration activity of neutrophil granulocytes under action of mitogen-induced immunocompetent blood cells, Peyer's patches, spleen and inguinal lymph was studied on a group of 55 male chinchilla rabbits with experimental model of widespread purulent peritonitis. It is established that the regulating action of immunocompetent cells on the migration of neutrophil leukocytes under the conditions of widespread purulent peritonitis is stable and widespread process, which is observed within 5 days of the postoperative period. The use of citoflavin and neoton during the postoperative period produces correction of the activity of immunocompetent cells, changing their properties in regulation of the migratory activity of neutrophil granulocytes. The effect is characteristic of both preparations and it is observed in all investigated organs, being manifested to a greater degree in immunocompetent cells of peripheral blood and Peyer's patches. Metabolic preparation citoflavin exhibits a more pronounced immunotropic action in comparison to neoton, which contains creatine phosphate.

Topics: Animals; Cardiotonic Agents; Cell Movement; Disease Models, Animal; Drug Combinations; Escherichia coli; Flavin Mononucleotide; Groin; Humans; Inosine Diphosphate; Lymph Nodes; Lymphocytes; Male; Monocytes; Neuroprotective Agents; Neutrophils; Niacinamide; Peritonitis; Peyer's Patches; Phosphocreatine; Postoperative Period; Rabbits; Spleen; Succinates; Suppuration

The experiment has been carried out on 55 male rabbits of chinchilla breed. For the first time in comparative aspect influence of preparations "Citoflavin" and "Neoton" on a phospholipid spectrum of liver mitochondrial membranes is studied at an experimental widespread purulent peritonitis. It is established that in the postoperative period "Citoflavin", which contains amber acid, influences on phospholipid structure of mitochondria of animals with a widespread purulent peritonitis by percentage decrease of lisophosphatides, level increase of polyglycerophosphatide and cardiolipin more effectively.

Topics: Animals; Cardiotonic Agents; Male; Mitochondria, Liver; Mitochondrial Membranes; Models, Animal; Peritonitis; Phosphocreatine; Phospholipids; Rabbits

Structural changes in the heart are studied at an experimental widespread purulent peritonitis. It was established that in 6 hours after initiation of peritonitis in a myocardium the severe disorders of blood circulation, interstitial edema, the phenomena of kariopyknosis, development of systemic toxic damages specifying in high speed at this disease were observed. One of the mechanism of their development is a energy deficiency. The proof of this is destructive changes of cardiomyocytes mitochondria. The comparative analysis of use of metabolic preparations "Citoflavin", containing amber acid, and "Neoton", containing phosphocreatine, for the purpose of pharmacological support of cardiac activity at an experimental widespread purulent peritonitis has revealed high efficiency of the preparation "Citoflavin". It caused more intensive decrease of inflammatory changes in a myocardium, preventing of cardiomyocytes necrobiosis, contribution to preservation of normal structure and growth of quantity of mitochondria.

Topics: Animals; Cardiotonic Agents; Disease Models, Animal; Heart Diseases; Mitochondria, Heart; Myocardium; Organs at Risk; Peritonitis; Phosphocreatine; Photomicrography; Rabbits; Severity of Illness Index; Suppuration; Treatment Outcome

Experiments performed in a group of 55 chinchilla rabbits showed that the development of widespread purulent peritonitis is accompanied by the expressed changes of protein-lipid structure of liver mitochondria. A comparative analysis of the application of metabolic preparations citoflavin and neoton for the purpose of eliminating pathological deviations in the structure of liver mitochondrian has been carried out. The application of citoflavin more effectively prevents decrease in total phospholipids and cholesterol in mitochondrial membranes. In combination with stimulation of an increase in the amount of protein, this points to high protective properties of this preparation which allow the functional potential of liver mitochondria to be retained under conditions of experimental widespread purulent peritonitis.

Topics: Animals; Cholesterol; Drug Combinations; Flavin Mononucleotide; Inosine Diphosphate; Male; Mitochondria, Liver; Mitochondrial Membranes; Niacinamide; Peritonitis; Phosphocreatine; Phospholipids; Proteins; Rabbits; Succinates; Suppuration

The research was performed in 55 male chinchilla rabbits. For the first time the effect of metabolic preparations "citoflavin" and "neoton" of the protein-lipid spectrum of blood was studied in experimental diffuse purulent peritonitis. The development of diffuse purulent peritonitis caused negative changes in blood lipid-transport system which resulted in a decreased blood protein level and high density lipoproteins (HDL) and growth of triglycerides. In the HDL phospholipid spectrum the pathological changes are characterized by an increased lisophosphotide content and compensatory growth of the level of poliglycerophosphatides. A comparative analysis has shown that both preparations possess unidirectional action which is more pronounced in "citoflavin" than in "neoton".

Topics: Animals; Biological Transport; Combined Modality Therapy; Drug Combinations; Drug Monitoring; Flavin Mononucleotide; Infusions, Intravenous; Inosine Diphosphate; Laparotomy; Lipid Metabolism; Male; Models, Animal; Niacinamide; Peritoneal Lavage; Peritonitis; Phosphocreatine; Protective Agents; Rabbits; Research Report; Succinates; Suppuration; Time Factors; Treatment Outcome; Vitamins

Experiments on 55 male chinchilla rabbits with model widespread purulent peritonitis have been performed for determinig structural changes in adrenal glands with the aid of optical microscopy. The introduction of aerobic-anaerobic culture of E. Coli and B. Fragilis into the abdominal cavity causes expressed structural changes in parenchyma of adrenal glands within 6 hours. It is established for the first time that the administration of metabolic drugs citoflavin (containing succinic acid) and neoton (containing creatine phosphate) prevents the development of pathological structural changes in adrenal glands under conditions of experimental widespread purulent peritonitis.

Topics: Adrenal Glands; Animals; Bacteroides fragilis; Bacteroides Infections; Escherichia coli; Escherichia coli Infections; Injections, Intravenous; Male; Microscopy; Microtomy; Paraffin Embedding; Peritonitis; Phosphocreatine; Rabbits; Succinic Acid; Suppuration

The functional activity of mitochondria of the muscular coat of small intestine (MCSI) has been studied in the normal state and under experimental widespread purulent peritonitis (WPP) conditions. The experiments have been carried out on a group of 55 male rabbits of chinchilla breed. It is established that, as a result of the WPP development, the functional activity of mitochondria in MCSI considerably decreases. The comparative analysis of the efficiency of metabolic drugs cytoflavin and neoton showed advantage of the citoflavin preparation, the administration of which allowed the indices of mitochondria in intact animals to be exceeded on the fifth day of postoperative period. The research results show expediency of a complex treatment of WPP using cytoflavin preparation for the normalization of biological oxidation processes and elimination of enteric insufficiency.

Topics: Animals; Bacillus; Drug Combinations; Escherichia coli; Flavin Mononucleotide; Injections, Intraperitoneal; Injections, Intravenous; Inosine Diphosphate; Intestine, Small; Male; Mitochondria; Muscle, Smooth; Niacinamide; Oxidative Phosphorylation; Peritonitis; Phosphocreatine; Rabbits; Succinates; Suppuration

One of the most powerful damaging factors during inflammation is hyperactivation of the free-radical oxidation processes. The condition of lipid peroxidation (LPO) and antioxidant activity (AOA) systems in liver has been studied in experiment on 55 male Chinchilla rabbits with widespread purulent peritonitis (WPP) model. The development of WPP leads to expressed of free-radical oxidation processes in liver that is manifested by systemic accumulation of toxic LPO products and a decrease in the activity of antioxidants. Comparative study of the influence of metabolic drugs citoflavin and neoton on the LPO and AOA systems has been conducted. It is established that citoflavin (containing succinic acid) exhibits more pronounced antioxidant properties, provides effective and fast restoration of the balance of LPO and AOA processes, and increases the speed of elimination of free radical.

Topics: Animals; Antioxidants; Cardiotonic Agents; Disease Models, Animal; Drug Combinations; Flavin Mononucleotide; Free Radicals; Inosine Diphosphate; Lipid Peroxidation; Liver; Male; Niacinamide; Peritonitis; Phosphocreatine; Rabbits; Succinates