phosphocreatine and Autolysis

The changes in 16 cerebral metabolites produced by cardiac arrest and subsequent room temperature autolysis were studied using high-resolution proton nuclear magnetic resonance spectroscopy. Biopsies of rabbit cerebral cortex, cerebral white matter, and cerebellum were quantitatively analyzed for acetate, alanine, gamma-aminobutyric acid, creatine, glutamate, glycine, inositol, lactate, N-acetylaspartate, phosphocreatine, succinate, taurine, and threonine. Of these, N-acetylaspartate and the total creatine pool are the best candidates for use as concentration reference standards linking in vitro to in vivo 1H nuclear magnetic resonance measurements. Both changed little immediately after death, and they varied in a distinctive way among cortex, white matter, and cerebellum.

Topics: 3-Hydroxybutyric Acid; Acetates; Amino Acids; Animals; Autolysis; Brain; Cerebellum; Cerebral Cortex; Creatine; Female; Hydroxybutyrates; Inositol; Lactates; Lactic Acid; Magnetic Resonance Spectroscopy; Phosphocreatine; Postmortem Changes; Rabbits; Succinates; Succinic Acid

Topics: Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphate; Anesthesia; Animals; Autolysis; Blood Pressure; Brain; Brain Chemistry; Carbon Dioxide; Carbon Radioisotopes; Cerebral Cortex; Cerebrovascular Circulation; Freezing; Glucose; In Vitro Techniques; Ketoglutaric Acids; Lactates; Male; Methods; Oxygen Consumption; Phenobarbital; Phosphocreatine; Pyruvates; Rats; Time Factors

Topics: Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphate; Anesthesia; Animals; Autolysis; Blood Pressure; Brain; Brain Chemistry; Carbon Dioxide; Creatine; Energy Metabolism; Freezing; Glucose; Hydrogen-Ion Concentration; Ketoglutaric Acids; Lactates; Methods; Mice; Mice, Inbred Strains; Nitrogen; Oxygen Consumption; Phosphocreatine; Pyruvates