pheophytin-a and Hemolysis

Novel nanohybrids consisting of nano-Ag, chitosan, lipids and phyto-compounds (chlorophyll a and curcumin) have been achieved through a simple bottom-up strategy, resulting in stable (ZP=-30.9mV) and spherical-shaped nano-entities with size <200nm (estimated by AFM analysis and DLS measurements). The formation of these biohybrids was monitored by absorption and emission spectroscopy, exploiting the spectral fingerprint of chlorophyll a. The bio-performances of these hybrid materials such as: high antioxidant activity (96.63%), strong biocidal properties against Escherichia coli ATCC 8738 (exhibiting an inhibition zone diameter of 32mm), hemocompatibility, in vitro cytotoxicity against HT-29 cancer cells and no toxicity to normal cells (in the biohybrid concentration range of 5.7-17%), make them promising candidates in bio-applications (antimicrobial and antioxidant coating, cancer treatment).

Topics: Animals; Anti-Bacterial Agents; Antioxidants; Biological Products; Cell Death; Cell Shape; Cell Survival; Chlorophyll; Chlorophyll A; Escherichia coli; Folic Acid; Hemolysis; HT29 Cells; Humans; Metal Nanoparticles; Mice; Microbial Sensitivity Tests; Microscopy, Atomic Force; Particle Size; Silver; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared; Static Electricity