phenylmercuric-acetate has been researched along with Lung-Diseases--Obstructive* in 1 studies
1 other study(ies) available for phenylmercuric-acetate and Lung-Diseases--Obstructive
Article | Year |
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Identification of a type V collagen-degrading enzyme from human sputum.
An enzyme capable of degrading type V collagen was found in the sputum of patients with chronic obstructive pulmonary disease (COPD). This enzyme was found in a latent form and was activated by 4-aminophenylmercuric acetate (APMA). Molecular sieve chromatography on Ultrogel AcA-34 and affinity chromatography on type V collagen-Sepharose were successful in separating type V from type I collagenolytic activity. The enzyme became active after affinity chromatography and no longer required activation with APMA to manifest activity. It was found to be a metalloproteinase by virtue of its inhibition by ethylene-diaminetetraacetic acid and phenanthroline but not by phenylmethanesulfonyl fluoride or N-ethylmaleimide. Because degradation of matrix proteins is felt to be important in the pathogenesis of COPD, it is possible that the selective degradation of type V collagen by this enzyme may play a role in the development of COPD. Topics: Chromatography, Affinity; Chromatography, Gel; Collagen; Electrophoresis, Polyacrylamide Gel; Endopeptidases; Humans; Lung Diseases, Obstructive; Metalloendopeptidases; Microbial Collagenase; Phenylmercuric Acetate; Sputum | 1983 |