phenylephrine-hydrochloride and Escherichia-coli-Infections

Here we report the draft genome sequence of Escherichia coli strain HN503E1II isolated from a nasal sample of an abattoir worker in Cameroon.. Bacterial genomic DNA of E. coli HN503E1II was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using the Qiagen CLC Genomics Workbench. The assembled contigs were annotated and antibiotic resistance genes, virulence factors, plasmids and the sequence type (ST) were identified.. The genome comprised a circular chromosome of 4 674201bp, with a 50.78% G+C content and several resistance genes associated with resistance to β-lactams, fluoroquinolones, aminoglycosides, sulphonamides, trimethoprim, macrolides and tetracycline. The isolate was assigned to ST10 with 100% identity among the seven housekeeping genes. In silico analysis also revealed the presence six plasmid types and one virulence factor.. The genome sequence reported here will provide valuable information for a better understanding of the genetic structure of the E. coli genome in Africa.

Topics: Abattoirs; Anti-Bacterial Agents; beta-Lactamases; Cameroon; DNA, Bacterial; Drug Resistance, Multiple, Bacterial; Escherichia coli; Escherichia coli Infections; Female; Genome, Bacterial; Humans; Nose; Plasmids; Virulence Factors; Whole Genome Sequencing

Topics: Abortion, Septic; Adult; Bacteremia; Critical Care; Disseminated Intravascular Coagulation; Escherichia coli; Escherichia coli Infections; Female; Humans; Nose; Pregnancy; Purpura Fulminans; Shock, Septic; Skin Diseases

Multiplex PCR was used to detect genes encoding selected virulence determinants associated with strains of Escherichia coli with K1 antigen (K1(+)) and non-K1 E. coli (K1(-)). The prevalence of the fimA, fimH, sfa/foc, ibeA, iutA and hlyF genes was studied for 134 (67 K1(+) and 67 K1(-)) E. coli strains isolated from pregnant women and neonates. The fimA gene was present in 83.6 % of E. coli K1(+) and in 86.6 % of E. coli K1(-) strains. The fimH gene was present in all tested E. coli K1(+) strains and in 97.0 % of non-K1 strains. E. coli K1(+) strains were significantly more likely to possess the following genes than E. coli K1(-) strains: sfa/foc (37.3 vs 16.4 %, P = 0.006), ibeA (35.8 vs 4.5 %, P<0.001), iutA (82.1 vs 35.8 %, P<0.001) and hlyF (28.4 vs 6.0 %, P<0.001). In conclusion, E. coli K1(+) seems to be more virulent than E. coli K1(-) strains in developing severe infections, thereby increasing possible sepsis or neonatal bacterial meningitis.

Topics: Antigens, Bacterial; Bacterial Capsules; Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Feces; Female; Gene Expression Regulation, Bacterial; Humans; Nose; Polymerase Chain Reaction; Polysaccharides, Bacterial; Pregnancy; Vagina; Virulence; Virulence Factors

The results of oral vaccination of 388 calves with herd-specific vaccines against E. coli are described. Innocuity was optimal, whereas the potency was estimated not as efficient as parenteral vaccination of the dam (in 22% of the cases), i.e., transient diseases in vaccinated calves occurred there. Further the results of intranasal vaccination of 496 cattle against salmonellosis are reported. Salmonella excretion in the faeces was stopped within 1-3 weeks post vaccination in 75% of the herds. In one case it took 3 months to reach this effect. A few permanent excretor cattle remained in two other cases.

Topics: Administration, Oral; Animals; Bacterial Vaccines; Cattle; Cattle Diseases; Escherichia coli Infections; Injections, Intravenous; Nose; Salmonella Infections, Animal; Vaccination

Topics: Aged; Arm; Bacterial Infections; Ear Diseases; Ear, External; Escherichia coli Infections; Female; Foot; Foot Diseases; Gangrene; Hand; Humans; Hypotension; Ischemia; Klebsiella Infections; Leg; Male; Middle Aged; Nose; Nose Diseases; Pneumococcal Infections; Pseudomonas Infections; Sepsis; Thrombosis

Topics: Anal Canal; Escherichia coli; Escherichia coli Infections; Female; Humans; Infant, Newborn; Infant, Newborn, Diseases; Infant, Premature, Diseases; Labor, Obstetric; Nose; Pregnancy

Topics: Child; Epidemics; Escherichia coli; Escherichia coli Infections; Humans; Infant; Infant, Newborn; Infant, Newborn, Diseases; Nose; Novobiocin; Nurseries, Infant