phenylephrine-hydrochloride and Central-Nervous-System-Neoplasms

Topics: Adolescent; Adult; Aged; Aged, 80 and over; Anatomic Landmarks; Central Nervous System Neoplasms; Cerebrospinal Fluid Rhinorrhea; Endoscopy; Epistaxis; Female; Humans; Male; Middle Aged; Natural Orifice Endoscopic Surgery; Nose; Postoperative Complications; Prospective Studies; Retrospective Studies; Sphenoid Sinus; Young Adult

The collection of the greatest possible amount of pathologic tissue is of paramount importance in neurosurgery to achieve the most accurate histopathologic diagnosis, to perform all of the necessary biomolecular tests on the pathologic specimen, and to collect biological material for basic or translational science studies. This problem is particularly relevant in pituitary surgery because of the possible small size and soft consistency of tumors, which make them suitable for removal through suction, reducing the amount of available pathologic tissue. To solve this issue, we adopted a filter connected to the suction tube, which allows the surgeon to collect all of the tissue aspirated during surgery.. Our experience of 1734 endoscopic endonasal procedures, performed adopting this device since 1998 to December 2015, has been revised to assess its advantages and limitations.. This system is easy-to-use, does not impair the surgical maneuvers, and does not add any relevant cost to the surgery. The tissue collected through the filter proved useful for diagnostic histologic and biomolecular analyses and for research purposes, without any relevant artifacts as a result of this method of collection.. The use of a filter has allowed us to obtain the greatest amount possible of pathologic tissue at each surgery. This surgical material has revealed to be helpful both for diagnostic and basic science purposes. The use of the filter has proven to be of particular importance for microadenomas, soft tumors, and supradiaphragmatic or skull base lesions with heterogeneous features, improving the accuracy of histopathologic diagnosis.

Topics: Adenoma; Central Nervous System Cysts; Central Nervous System Neoplasms; Chondrosarcoma; Chordoma; Cranial Fossa, Posterior; Craniopharyngioma; Glioma; Humans; Hypothalamic Neoplasms; Meningeal Neoplasms; Meningioma; Natural Orifice Endoscopic Surgery; Neuroendoscopy; Nose; Pituitary Neoplasms; Plasmacytoma; Retrospective Studies; Skull Base Neoplasms; Suction

Topics: Central Nervous System Neoplasms; Female; Glioma; Humans; Infant, Newborn; Nose

Phenotypic parallels and genetic evidence from comparative mapping suggest that the murine Small eye (Sey) and human aniridia (AN) disorders are homologous. This report describes the isolation of a murine embryonic cDNA that is structurally homologous to the AN cDNA were recently cloned. The murine cDNA detects a 2.7-kb transcript in the adult mouse eye and cerebellum and in human glioblastomas, suggesting a neuroectodermal involvement in the etiology of Sey/AN. Sequence comparison between the murine and the human cDNAs revealed extensive homology in nucleotide sequence (greater than 92%) and virtual identity at the amino acid level. None of the differing amino acids was located within the paired box and homeobox DNA-binding domains. These results provide evidence for a common molecular basis underlying the two genetic disorders and suggest that the Sey system would be an authentic model for human AN.

Topics: Amino Acid Sequence; Animals; Aniridia; Base Sequence; Brain; Central Nervous System Neoplasms; Cloning, Molecular; DNA; Eye; Eye Abnormalities; Gene Expression; Gene Expression Regulation, Neoplastic; Genes, Lethal; Humans; Mice; Molecular Sequence Data; Neural Crest; Nose; Organ Specificity; Regulatory Sequences, Nucleic Acid; RNA, Messenger; RNA, Neoplasm; Sequence Homology, Nucleic Acid; Tumor Cells, Cultured