phalloidine and Obesity

To investigate the effect of obesity induced by high fat diet on decidual reaction of endometrium in mice, and the effect of high fat treatment on decidual reaction of endometrial stromal cells.. Twelve 4-week-old healthy C57BL/6J female mice were randomly divided into high fat diet group and control group with 6 mice in each group. They were fed with high fat diet (22 kJ/g) or normal diet (16 kJ/g) for 12 weeks, respectively. The body weight of mice was measured every week. After feeding for 12 weeks, the body length and width of mice were measured, and the levels of fasting serum triglyceride and total cholesterol were determined. Then the mice were mated with healthy C57BL/6J male mice, and the uterine tissues were collected on the seventh day of pregnancy. The decidual cells and collagen fibers in mouse endometrium was observed by HE staining and Masson staining respectively. The expression of decidual reaction related proteins in mouse endometrium were detected by immunohistochemistry and Western blotting. Mouse endometrial stromal cells (mESCs) were isolated and treated with the oleic acid and palmitic acid. After feeding for 12 weeks, the body weight of mice in the high fat group was significantly higher than that in the control group (. Obesity induced by high fat diet and high fat treatment can impair the decidual reaction of endometrium and endometrial stromal cells in mice.

Topics: Animals; Azo Compounds; Body Weight; Bone Morphogenetic Proteins; Boron Compounds; Cholesterol; Collagen; Diet, High-Fat; Endometrium; Estradiol; Female; Homeobox A10 Proteins; Male; Mice; Mice, Inbred C57BL; Obesity; Oleic Acid; Palmitic Acid; Phalloidine; Pregnancy; Progesterone; Prostaglandin-Endoperoxide Synthases; Triglycerides