phalloidine and Body-Weight

To investigate the effect of obesity induced by high fat diet on decidual reaction of endometrium in mice, and the effect of high fat treatment on decidual reaction of endometrial stromal cells.. Twelve 4-week-old healthy C57BL/6J female mice were randomly divided into high fat diet group and control group with 6 mice in each group. They were fed with high fat diet (22 kJ/g) or normal diet (16 kJ/g) for 12 weeks, respectively. The body weight of mice was measured every week. After feeding for 12 weeks, the body length and width of mice were measured, and the levels of fasting serum triglyceride and total cholesterol were determined. Then the mice were mated with healthy C57BL/6J male mice, and the uterine tissues were collected on the seventh day of pregnancy. The decidual cells and collagen fibers in mouse endometrium was observed by HE staining and Masson staining respectively. The expression of decidual reaction related proteins in mouse endometrium were detected by immunohistochemistry and Western blotting. Mouse endometrial stromal cells (mESCs) were isolated and treated with the oleic acid and palmitic acid. After feeding for 12 weeks, the body weight of mice in the high fat group was significantly higher than that in the control group (. Obesity induced by high fat diet and high fat treatment can impair the decidual reaction of endometrium and endometrial stromal cells in mice.

Topics: Animals; Azo Compounds; Body Weight; Bone Morphogenetic Proteins; Boron Compounds; Cholesterol; Collagen; Diet, High-Fat; Endometrium; Estradiol; Female; Homeobox A10 Proteins; Male; Mice; Mice, Inbred C57BL; Obesity; Oleic Acid; Palmitic Acid; Phalloidine; Pregnancy; Progesterone; Prostaglandin-Endoperoxide Synthases; Triglycerides

Phalloidin, administered to male rats for 7 days (500 microgram per kg/day), increased the mean hepatic content of filamentous actin. Both bile flow and bile acid excretion diminished proportionally, whereas the bile-to-plasma ratios of [3H]inulin and [14C]sucrose increased significantly from 0.08 and 0.16 in controls to 0.37 and 0.69, respectively, in phalloidin-treated animals. Simultaneously, junctional permeability was altered as noted by the free penetration of ionic lanthanum into the zonula occludens and bile canaliculus. Freeze-fracture replicas of the junctional complex revealed rearrangements of the junctional elements and regions in which only a single element separated the canaliculus from the lateral intercellular space. These findings suggest that microfilaments influence the permeability of "tight junctions" between hepatocytes and that bile constituents might reflux from the canaliculus to the intercellular space in phalloidin-induced cholestasis.

Topics: Actins; Animals; Bile; Body Weight; Cholestasis; Cytoskeleton; Intercellular Junctions; Inulin; Liver; Male; Oligopeptides; Permeability; Phalloidine; Rats; Sucrose

The effectiveness of three plasma cleansing techniques (exchange transfusion, peritoneal dialysis and plasmapheresis) is studied in the treatment of phalloides intoxication. The severity of the latter is dependent upon the amount of toxin ingested, with can now be measured by radio-immunology, as well as the fixation of the toxin in the liver. Methods to ensure its elimination must be instituted as soon as possible. Thus in 43 patients who had consumed more than 50 g of fresh fungi, there were no deaths amongst those treated during the first 36 hours and 7 deaths out of 22 patients treated late. It is not yet possible to define the respective values of each method.

Topics: Adolescent; Adult; Agaricales; Aged; Amanita; Amanitins; Blood Transfusion; Body Weight; Child; Child, Preschool; Female; Humans; Male; Middle Aged; Mushroom Poisoning; Oligopeptides; Peritoneal Dialysis; Phalloidine; Radioimmunoassay; Time Factors