pf-4708671 and Reperfusion-Injury

It is not clear whether inhibition of p70 ribosomal S6 kinase 1 (S6K1) is neuroprotective in cerebral ischemia-reperfusion. Decreasing blood-brain barrier (BBB) disruption has been associated with a better neuronal outcome in cerebral ischemia. We hypothesized that inhibition of S6K1 would decrease BBB disruption and infarct size in the early stage of cerebral ischemia-reperfusion. Middle cerebral artery occlusion (MCAO) was performed in rats under isoflurane anesthesia with controlled ventilation. 75 mg/kg of PF-4708671, an S6K1 inhibitor, was administered intraperitoneally 15 min after MCAO. After 1 h of MCAO and 2 h of reperfusion, the transfer coefficient (K

Topics: Animals; Blood-Brain Barrier; Enzyme Inhibitors; Hemodynamics; Imidazoles; Infarction, Middle Cerebral Artery; Male; Permeability; Phosphorylation; Piperazines; Rats; Reperfusion Injury; Ribosomal Protein S6 Kinases; Signal Transduction; Time Factors