pervanadate and Necrosis

pervanadate has been researched along with Necrosis* in 1 studies

Other Studies

1 other study(ies) available for pervanadate and Necrosis

Article	Year
Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells. FEBS letters, 2001, Nov-16, Volume: 508, Issue:2 Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydrogen peroxide alone activated PLD in a dose- and time-dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan-1-ol, a PLD inhibitor, attenuated both pervanadate-induced necrosis and increase of intracellular Ca(2+). Removal of extracellular Ca(2+) inhibited pervanadate-induced necrosis by 50%. These results suggest that PLD activation mediates pervanadate-induced necrosis of VSMCs, which is at least partly due to Ca(2+) toxicity. Topics: Animals; Aorta; Butanols; Calcium; Cells, Cultured; Dose-Response Relationship, Drug; Enzyme Activation; Flow Cytometry; Hydrogen Peroxide; Muscle, Smooth, Vascular; Necrosis; Oxidative Stress; Phospholipase D; Rats; Time Factors; Vanadates	2001

Article

Year

Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells.

FEBS letters, 2001, Nov-16, Volume: 508, Issue:2

Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydrogen peroxide alone activated PLD in a dose- and time-dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan-1-ol, a PLD inhibitor, attenuated both pervanadate-induced necrosis and increase of intracellular Ca(2+). Removal of extracellular Ca(2+) inhibited pervanadate-induced necrosis by 50%. These results suggest that PLD activation mediates pervanadate-induced necrosis of VSMCs, which is at least partly due to Ca(2+) toxicity.

Topics: Animals; Aorta; Butanols; Calcium; Cells, Cultured; Dose-Response Relationship, Drug; Enzyme Activation; Flow Cytometry; Hydrogen Peroxide; Muscle, Smooth, Vascular; Necrosis; Oxidative Stress; Phospholipase D; Rats; Time Factors; Vanadates

2001