perlite and Staphylococcal-Skin-Infections

The preparation of ointments from natural compounds is essential for accelerating infected wounds. This study investigated the effects of topical uses of gold nanoparticles (Au)/perlite (Au/Perl) nanocomposites (NCs) by the help of Urtica dioica extract and its chitosan-capped derivative (Chit) on methicillin-resistant Staphylococcus aureus (MRSA)-infected wound healing in a mouse model. Furthermore, Au/Perl/Chit nanocomposite was prepared using protonated chitosan solution. The physicochemical properties of the as-synthesized nanocomposites were also investigated. The effects of Au/Perl/Chit NC were assessed by antibacterial, histopathological parameters as well as molecular evaluations. Then, they were compared with synthetic agent of mupirocin. The results revealed that Au/Perl NC was mesoporous and spherical in a range of 13-15 nm. Topical administration of Au/Perl/Chit ointment accelerated wound healing by reducing bacteria colonization and wound rate enhancing collagen biosynthesis and re-epithelialization, the expressions of IL-10, PI3K, AKT, bFGF, and COL1A genes, which is in agreement with the obtained results for mupirocin. In conclusion, the results strongly demonstrated that administration of ointments prepared from Au/Perl and Au/Perl/Chit nanocomposites stimulates MRSA-infected wound healing by decreasing the length of healing time and regulating PI3K/AKT/bFGF signaling pathway and is a promising candidate in stimulating MRSA-infected wound regeneration.

Topics: Aluminum Oxide; Animals; Anti-Bacterial Agents; Antioxidants; Cell Proliferation; Chitosan; Disease Models, Animal; Drug Compounding; Fibroblasts; Gold Compounds; Green Chemistry Technology; Male; Methicillin-Resistant Staphylococcus aureus; Mice; Mice, Inbred BALB C; Nanoparticles; Nanotechnology; NIH 3T3 Cells; Signal Transduction; Silicon Dioxide; Skin; Staphylococcal Skin Infections; Time Factors; Urtica dioica; Wound Healing