peptones and Swine-Diseases

The enteropooling activity of soya-peptone was studied in germfree piglets. The enteropooling activity was restricted to the proximal part of the jejunum and was found to be blocked by aspirin. The hypothesis that soya-peptone acts via an increased biosynthesis/release of prostanoids was further studied using guinea-pig lung parenchyma. In this tissue soya-peptone induces the release of TXB2, which was inhibited by indomethacin (3 microM). The involvement of cholinergic, histaminergic and serotonergic pathways in the soya-peptone-mediated response was excluded, using antagonists. The conclusion was reached that soya-peptone can stimulate/modulate the biosynthesis of cyclic eicosanoids and that it exerts its enteropooling activity via the same mechanism in the jejunum of piglets.

Topics: Animal Feed; Animals; Aspirin; Diarrhea; Eicosanoic Acids; Fatty Acids; Glycine max; Guinea Pigs; Jejunum; Lung; Peptones; Prostanoic Acids; Swine; Swine Diseases

Haemophilus pleuropneumoniae and Haemophilus parasuis were grown in a liquid medium containing tryptone and yeast extract. Whereas culture turbidity during exponential growth of either organism was proportional to the number of viable cells, continued incubation of stationary phase cultures was accompanied by a dramatic decrease in viability with no corresponding decrease in culture turbidity. It is concluded that the medium described should prove useful for the growth of porcine haemophili for use in experimental infections provided the stage of growth is given due consideration.

Topics: Animals; Culture Media; Haemophilus; Haemophilus Infections; Peptones; Swine; Swine Diseases