peptones and Hemolysis

About 60 characteristics have been investigated in 7 hemolyzing and 12 nonhemolyzing strains of L. monocytogenes. From these investigations resulted inter alia that the organism grows well under strictly anaerobic conditions, esculin is split at 45 degrees C,NH3 is produced from peptone, but not from arginin, and H2S can be traced by sufficiently sensitive methods. All strains possess a lipase, muramidase, and deoxyribonuclease, the hemolytic ones only also a lecithinase. Besides, the hemolytic strains only dispose of experimental virulence and of a CAMP factor-like agent. The experimental animal of choice seems to be the conjunctivally infected guinea pig in which a generalized infection develops.

Topics: Ammonia; Anaerobiosis; Arginine; Deoxyribonucleases; Esculin; Hemolysis; Hydrogen Sulfide; Lipase; Listeria monocytogenes; Muramidase; Peptones; Phospholipases; Temperature

Strain-dependent variations in the ability of Bordetella bronchiseptica to produce haemolysis on solid media exist. Haemolysis is strongest at an acid reaction and will not take place if the reaction is too alkaline. Peptone and glutamine inhibit haemolysis and favour growth. The vigorous growth rapidly produced an alkaline reaction which inhibits the haemolysis.

Topics: Amides; Amino Acids; Bordetella; Culture Media; Glutamine; Hemolysis; Hydrogen-Ion Concentration; Peptones; Time Factors

Topics: Agar; Agglutination Tests; Animals; Bile Acids and Salts; Carbohydrate Metabolism; Cholera; Culture Media; Diagnosis, Differential; Drug Resistance, Microbial; Erythrocytes; Hemolysis; Humans; Immune Sera; Methods; Microscopy, Phase-Contrast; Peptones; Polymyxins; Rosaniline Dyes; Serotyping; Sheep; Species Specificity; Specimen Handling; Staining and Labeling; Taurine; Vibrio

Topics: Agar; Animals; Blood; Culture Media; Environment; Fibrinolysin; Hemolysis; Humans; Meat; Peptones; Staphylococcus