peptones and Fibrosarcoma

The effect of syngeneic mouse peritoneal cells (PC) on the growth of four different transplantable tumours was studied in adoptive transfer experiments (Winn's test). PC from unstimulated mice did not influence the growth of a benzpyrene induced fibrosarcoma (BaF1) and a methylcholantrene induced mastocytoma (P815), but significantly enhanced the growth of a spontaneous adenocarcinoma (Sp4) and Lewis lung carcinoma (LL). PC induced by a single injection of thioglycollate did not influence, whereas PC elicited by proteose peptone markedly enhanced the growth of BaF1 fibrosarcoma. The enhancing effect of peptone induced PC was diminished by a single intraperitoneal dose (100 micrograms/mouse) of polyinosinic-polycytidylic acid (poly I:C) given after peptone injection. Transferring PC obtained after a single injection of poly I:C (100 micrograms intraperitoneally) resulted in retardation of growth of BaF1 fibrosarcoma and Sp4 adenocarcinoma or in a marked decrease in their take depending on the PC/tumour cell ratio. The effector cells involved in the protective effect proved to be different, using these two tumour models. Lewis lung carcinoma and P815 mastocytoma proved to be insensitive to poly I:C-stimulated PC.

Topics: Adenocarcinoma; Animals; Caseins; Cell Adhesion; Female; Fibrosarcoma; Immunization, Passive; Lung Neoplasms; Lymphocytes; Macrophages; Male; Mast-Cell Sarcoma; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred DBA; Neoplasm Transplantation; Neoplasms, Experimental; Peptide Fragments; Peptones; Peritoneal Cavity; Poly I-C; Thioglycolates

Rats and mice bearing transplanted chemically induced neoplasms have defective macrophage infiltration of inflammatory sites distant to the tumor. The defect limits concurrently accumulation of macrophages within the tumor, raising dramatically the tumor to macrophage cell ratio. The defect may not compromise host surveillance because it requires relatively large numbers of tumor cells. The abnormality does not appear to result from circulating monocyte depletion, defective monocyte chemotaxis, or the traffic of monocytes into the tumor.

Topics: 9,10-Dimethyl-1,2-benzanthracene; Animals; Cell Movement; Cell Transformation, Neoplastic; Chemotaxis; Fibrosarcoma; Inflammation; Macrophages; Monocytes; Neoplasm Transplantation; Peptones; Rats

Topics: Animals; Concanavalin A; Cytotoxicity, Immunologic; Dextrans; Erythrocytes; Fibrosarcoma; Lipopolysaccharides; Macrophages; Mannose; Peptones; Phytohemagglutinins; Rats; Sheep; Solubility

Previous studies have indicated, that, after in vitro incubation of antigen with macrophages, the "processed" antigen preferentially induces cell-mediated immunity. To investigate this phenomenon with tumor antigens, mycobacteria-stimulated macrophages were incubated with irradiated syngeneic EMT6 tumor cells for varying lengths of time and injected into normal mice. On subsequent challenge with EMT6, there was a significant increase in protection in mice immunized with macrophage-processed tumor antigen over control animals. Mineral oil-stimulated macrophages were also capable of processing irradiated EMT6, but macrophages induced by thioglycollate or proteose peptone were not. Freeze-thawed mycobacteria-stimulated macrophages were nearly as effective as viable macrophages in processing tumor antigen, but heat-treated macrophages lost this capacity. The immunity generated was specific and could be passively transferred by immune cells but not by immune serum. The results indicate that incubation of tumor antigen with appropriately activated macrophages leads to the enhanced induction of immunity to the tumor. Macrophage enzymes may degrade tumor antigens to fragments with few antigenic determinants that preferentially induce cell-mediated immunity.

Topics: Animals; Antigens, Neoplasm; Cell Line; Epitopes; Female; Fibrosarcoma; Immunity, Cellular; Immunization, Passive; Immunotherapy; Macrophages; Male; Mammary Neoplasms, Experimental; Mice; Mice, Inbred BALB C; Mineral Oil; Mycobacterium phlei; Neoplasm Transplantation; Neoplasms, Experimental; Peptones; Thioglycolates; Transplantation, Isogeneic