peptide-31d and Disease-Models--Animal

peptide-31d has been researched along with Disease-Models--Animal* in 1 studies

Other Studies

1 other study(ies) available for peptide-31d and Disease-Models--Animal

Article	Year
Analysis of SIV-specific CTL in the rhesus macaque model of AIDS: the use of simian fibroblasts as an alternative source of target cells for chromium release assays. Journal of immunological methods, 2001, Dec-01, Volume: 258, Issue:1-2 The simian immunodeficiency virus (SIV) model of AIDS is widely used for the development of human immunodeficiency virus (HIV) vaccine strategies, particularly for the analysis of correlates of protective immunity. As it is not always possible to establish autologous B-lymphoblastoid cell lines (B-LCL) for use as targets in the analysis of cytotoxic T cell (CTL) activity, we have compared B-LCL with primary simian skin cells. Using a well-defined SIV gag-encoded CTL epitope restricted by Mamu A*01 major histocompatibility complex (MHC) class I, we have shown that peripheral blood mononuclear cells (PBMC) from vaccinated and infected macaques can kill MHC class I-matched skin fibroblasts presenting the cognate epitope but that skin fibroblasts are a less sensitive target than B-LCL for the detection of CTL. Topics: Acquired Immunodeficiency Syndrome; Animals; Antigens, Viral; B-Lymphocytes; Cell Line; Chromium; Cytotoxicity Tests, Immunologic; Disease Models, Animal; Fibroblasts; Gene Products, gag; Histocompatibility Antigens Class I; Humans; Macaca mulatta; Nucleoproteins; Simian Acquired Immunodeficiency Syndrome; Simian Immunodeficiency Virus; T-Lymphocytes, Cytotoxic	2001

Article

Year

Analysis of SIV-specific CTL in the rhesus macaque model of AIDS: the use of simian fibroblasts as an alternative source of target cells for chromium release assays.

Journal of immunological methods, 2001, Dec-01, Volume: 258, Issue:1-2

The simian immunodeficiency virus (SIV) model of AIDS is widely used for the development of human immunodeficiency virus (HIV) vaccine strategies, particularly for the analysis of correlates of protective immunity. As it is not always possible to establish autologous B-lymphoblastoid cell lines (B-LCL) for use as targets in the analysis of cytotoxic T cell (CTL) activity, we have compared B-LCL with primary simian skin cells. Using a well-defined SIV gag-encoded CTL epitope restricted by Mamu A*01 major histocompatibility complex (MHC) class I, we have shown that peripheral blood mononuclear cells (PBMC) from vaccinated and infected macaques can kill MHC class I-matched skin fibroblasts presenting the cognate epitope but that skin fibroblasts are a less sensitive target than B-LCL for the detection of CTL.

Topics: Acquired Immunodeficiency Syndrome; Animals; Antigens, Viral; B-Lymphocytes; Cell Line; Chromium; Cytotoxicity Tests, Immunologic; Disease Models, Animal; Fibroblasts; Gene Products, gag; Histocompatibility Antigens Class I; Humans; Macaca mulatta; Nucleoproteins; Simian Acquired Immunodeficiency Syndrome; Simian Immunodeficiency Virus; T-Lymphocytes, Cytotoxic

2001