pepstatin and Struma-Ovarii

High concentration of human calcitonin (hCT) was found in an ovarian carcinoid by radioimmunoassay. The hCT value was not affected by the presence of protease inhibitors. To confirm the presence of hCT in an ovarian carcinoid, hCT was isolated by the Baghdiantz method. The molecular weight of the ovarian hCT was determined using Sephadex G-75 gel filtration. Though the molecular weight of the ovarian hCT was variable, 90% corresponded to that of the authentic hCT. The carcinoid cells were examined by immunoperoxidase techniques. Those composed of strumal and trabecular structure were all argyrophilic, but hCT was only found in strumal structure. Significant concentrations of hCT were also found in ovarian cancers.

Topics: Aprotinin; Benzamidines; Biomarkers, Tumor; Calcitonin; Carcinoid Tumor; Chromatography, Gel; Edetic Acid; Female; Gabexate; Guanidines; Humans; Ovarian Neoplasms; Pepstatins; Prostaglandins E; Radioimmunoassay; Struma Ovarii