peplomycin and Myosarcoma

Tumor cells derived from 13 different individual human tumors were plated in a colony forming monolayer assay. The effect of bleomycin and peplomycin on colony formation was assessed in normothermic conditions and after a hyperthermic treatment at 40.5 degrees C for 2 h at the beginning of the culture. In three out of the 13 tumor samples (two colon carcinomas, one malignant melanoma), hyperthermic incubation resulted in a thermal enhancement of the effects of bleomycin and peplomycin. In addition, human bone marrow progenitor cells (CFU-C) were subjected to the same procedure. Peplomycin proved to be less toxic to CFU-C than bleomycin. In samples from eight different donors, homogeneous dose-response curves were observed. There was no difference between normo- and hyperthermic incubation.

Topics: Bleomycin; Cell Survival; Colonic Neoplasms; Colony-Forming Units Assay; Dose-Response Relationship, Drug; Gallbladder Neoplasms; Hematopoietic Stem Cells; Hot Temperature; Humans; Lung Neoplasms; Melanoma; Myosarcoma; Neoplastic Stem Cells; Peplomycin; Tumor Stem Cell Assay

The cytotoxic effect of bleomycin and peplomycin was compared using a methylcellulose monolayer assay for the cultivation of human tumor cells. In 3 out of 4 samples from human malignant melanomas peplomycin proved to be more cytotoxic than bleomycin. Peplomycin was more cytotoxic than bleomycin in 1 of 5 myosarcoma samples, whereas 2 samples from squamous cell carcinomas of the lung showed identical dose response curves. In 1 carcinoma of the gall bladder peplomycin was more toxic than bleomycin.

Topics: Bleomycin; Cells, Cultured; Colonic Neoplasms; Colony-Forming Units Assay; Dose-Response Relationship, Drug; Humans; Lung Neoplasms; Melanoma; Myosarcoma; Peplomycin; Tumor Stem Cell Assay; Urinary Bladder Neoplasms