peplomycin and Drug-Hypersensitivity

An allergic reaction to peplomycin was observed in a patient with cervical uterine cancer who had previously been treated with peplomycin. A positive Prausnitz-Küstner test and its elimination after heat treatment of the serum showed the production of anti-peplomycin IgE antibody. Peplomycin was coupled to a paper disc and a sensitive radioallergosorbent test for peplomycin was developed to quantitate the antibody. Patient serum IgE and IgG were purified by DE52 column chromatography; the IgE fraction contained binding activity to peplomycin. A competition test revealed that the antibody bound to both peplomycin and bleomycin. DNA, RNA, and mononucleotides had no effect on antibody binding, but the antibody inhibited peplomycin's activity.

Topics: Aged; Antibody Specificity; Bleomycin; Combined Modality Therapy; Cross Reactions; Drug Hypersensitivity; Female; Humans; Immunoglobulin E; Immunoglobulin G; Intradermal Tests; Peplomycin; Uterine Cervical Neoplasms