peperomin-e and Inflammation

Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Atherosclerosis; Benzodioxoles; Endothelium, Vascular; Humans; Inflammasomes; Inflammation; Mice; NLR Family, Pyrin Domain-Containing 3 Protein; Recombinant Proteins; Vimentin

Peperomin E (PepE) is a type of secolignan, a major component of the plant Peperomia dindygulensis. It has been shown to exert anti-inflammatory effects; however, the effects of PepE on human atherosclerosis remain unexplored. In the study, we investigated the role of PepE in high fat diet (HFD) induced atherosclerosis using apolipoprotein E defcient (ApoE

Topics: Animals; Apolipoproteins E; Atherosclerosis; Benzodioxoles; Cell Survival; Diet, High-Fat; Inflammation; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; NLR Family, Pyrin Domain-Containing 3 Protein; Reactive Oxygen Species; Signal Transduction

The transcription factor nuclear factor kappaB (NF-kappaB) induces the expression of various inflammatory genes. In the common NF-kappaB signaling pathway, peperomin E and 2,6-didehydropeperomin B inhibited IkappaB degradation upon stimulation with TNF-alpha or interleukin-1. Consistent with these results, peperomin E and 2,6-didehydropeperomin B blocked the TNF-alpha-induced activation of IkappaB kinase, while they had no direct effect on the IkappaB kinase activity. Our present results clearly demonstrate that peperomins inhibit the NF-kappaB signaling pathway by blocking IkappaB kinase activation.

Topics: Anti-Inflammatory Agents; Benzodioxoles; Cell Line, Tumor; Chemistry, Pharmaceutical; Drug Design; Gene Expression Regulation; Humans; Inflammation; Interleukin-1; Models, Chemical; NF-kappa B; Phosphorylation; Signal Transduction; Structure-Activity Relationship; Tumor Necrosis Factor-alpha