peoniflorin and Rhinitis--Allergic

Paeoniflorin (PF), one of the main effective ingredients from the root of Paeonia lactiflora Pall., was reported to possess antitumor, anti-inflammatory, and antiallergic properties. However, the roles of PF in activated human mast cell line, HMC-1 cells, have not yet been elucidated. Thus, the aim of this study was to examine the antiallergic and anti-inflammatory effects of PF on phorbol-12-myristate 13-acetate plus calcium ionophore (PMACI)-induced human mast cells and to identify the mechanism responsible for these effects. Our results demonstrated that pretreatment with PF effectively attenuated PMACI-induced production of tumor necrosis factor-α and interleukin 1β in HMC-1 cells. In addition, PF significantly suppressed PMACI-induced histamine release and caspase-1 activation in HMC-1 cells. Furthermore, PF prevented the activation of nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways in activated HMC-1 cells. In conclusion, we showed for the first time that PF attenuated the mast cell-mediated allergic inflammatory response through suppressing the NF-κB and MAPK signaling pathways.

Topics: Analysis of Variance; Anti-Inflammatory Agents, Non-Steroidal; Calcium Ionophores; Caspase 1; Caspase Inhibitors; Cell Line, Tumor; Cell Survival; Glucosides; Histamine Antagonists; Humans; Interleukin-1beta; Mast Cells; Mitogen-Activated Protein Kinases; Monoterpenes; NF-kappa B; Paeonia; Plant Extracts; Rhinitis, Allergic; Signal Transduction; Tetradecanoylphorbol Acetate; Tumor Necrosis Factor-alpha