pelargonidin and Inflammation

The present study investigates the effect of strawberry antioxidants in beverage form on meal-induced postprandial inflammatory and insulin responses in human subjects. Overweight adults (n 24) consumed a high-carbohydrate, moderate-fat meal (HCFM) accompanied by either a strawberry or a placebo beverage in a cross-over design. Postprandial changes in plasma anthocyanins, their metabolites, insulin, glucose and inflammatory markers were assessed for 6 h. The postprandial concentrations of pelargonidin sulfate and pelargonidin-3-O-glucoside were significantly increased when the strawberry beverage was consumed concurrently with the HCFM compared with the placebo beverage (P < 0·001). The strawberry beverage significantly attenuated the postprandial inflammatory response as measured by high-sensitivity C-reactive protein and IL-6 (P < 0·05) induced by the HCFM. It was also associated with a reduction in postprandial insulin response (P < 0·05). Collectively, these data provide evidence for favourable effects of strawberry antioxidants on postprandial inflammation and insulin sensitivity.

Topics: Adult; Anthocyanins; Beverages; C-Reactive Protein; California; Cross-Over Studies; Female; Fragaria; Glucosides; Humans; Inflammation; Insulin; Interleukin-6; Male; Middle Aged; Oxidants; Oxidative Stress; Placebos; Postprandial Period; Single-Blind Method; Sulfates; Time Factors

Topics: Animals; Anthocyanins; Anti-Inflammatory Agents; Caco-2 Cells; Colitis; Fatty Liver; Hep G2 Cells; Humans; Inflammation; Macrophages; Methylation; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; RAW 264.7 Cells; Receptors, Aryl Hydrocarbon

Previous reports suggest that human endothelial cells-derived PolyPhosphate (PolyP) is one of the pro-inflammatory mediators. As a well-known red pigment and found in plants, Pelargonidin (PEL) has been known to have several biological activates which are beneficial for human health. This study was undertaken to investigate whether PEL can modulate PolyP-mediated inflammatory responses in human umbilical vein endothelial cells (HUVECs) and in mice. The anti-inflammatory activities of PEL were determined by measuring permeability, leukocytes adhesion and migration, and activation of pro-inflammatory proteins in PolyP-activated HUVECs and mice. In addition, the beneficial effects of PEL on survival rate in PolyP-injected mice. We found that PEL inhibits PolyP-mediated barrier disruption, the expressions of cell adhesion molecules, and leukocyte to HUVEC adhesion/migration. Interestingly, PolyP-induced NF-κB activation and the productions of TNF-α and IL-6 were inhibited by PEL in HUVECs. These anti-inflammatory functions of PEL were confirmed in PolyP injected mice. These results suggest that PEL have therapeutic potential for various systemic inflammatory diseases.

Topics: Animals; Anthocyanins; Anti-Inflammatory Agents, Non-Steroidal; Capillary Permeability; Cell Adhesion; Cell Movement; Cells, Cultured; Human Umbilical Vein Endothelial Cells; Humans; Inflammation; Interleukin-6; Leukocytes; Male; Mice; Mice, Inbred C57BL; NF-kappa B; Polyphosphates; Tumor Necrosis Factor-alpha