pectins and Poisoning

Topics: Animal Feed; Animals; Anticholesteremic Agents; Antitoxins; Ascorbic Acid; Azo Compounds; Carbohydrates; Cellulose; Cyclamates; Diet; Dietary Carbohydrates; Heptoses; Humans; Medicago sativa; Methyl Ethers; Mice; Pectins; Plants; Poaceae; Poisoning; Polysaccharides; Polysorbates; Rats; Sodium; Surface-Active Agents; Triazines

In the copper metallurgy workplace air is polluted with condensation aerosols, which a significant fraction of is presented by copper oxide particles<100 nm. In the scientific literature, there is a lack of their in vivo toxicity characterization and virtually no attempts of enhancing organism's resistance to their impact. A stable suspension of copper oxide particles with mean (±SD) diameter 20±10 nm was prepared by laser ablation of pure copper in water. It was being injected intraperitoneally to rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week up to 19 injections. In parallel, another group of rats was so injected with the same suspension against the background of oral administration of a "bio-protective complex" (BPC) comprising pectin, a multivitamin-multimineral preparation, some amino acids and fish oil rich in ω-3 PUFA. After the termination of injections, many functional and biochemical indices for the organism's status, as well as pathological changes of liver, spleen, kidneys, and brain microscopic structure were evaluated for signs of toxicity. In the same organs we have measured accumulation of copper while their cells were used for performing the Random Amplification of Polymorphic DNA (RAPD) test for DNA fragmentation. The same features were assessed in control rats infected intraperitoneally with water with or without administration of the BPC. The copper oxide nanoparticles proved adversely bio-active in all respects considered in this study, their active in vivo solubilization in biological fluids playing presumably an important role in both toxicokinetics and toxicodynamics. The BPC proposed and tested by us attenuated systemic and target organs toxicity, as well as genotoxicity of this substance. Judging by experimental data obtained in this investigation, occupational exposures to nano-scale copper oxide particles can present a significant health risk while the further search for its management with the help of innocuous bioprotectors seems to be justified.

Topics: Air Pollutants, Occupational; Amino Acids; Animals; Brain; Copper; DNA Damage; Fatty Acids, Omega-3; Heavy Metal Poisoning; Kidney; Liver; Nanoparticles; Pectins; Poisoning; Protective Agents; Rats; Tissue Distribution; Vitamins

The pectin-vitamin composition (PVC) and its complex with active carbon (AC) was investigated for use as an oral sorbent to protect radionuclide (Sr-85, Cs-137) accumulation. PVC is composed of the natural mixture of cellulose, pectin (Ca-salt), flavonoides, vitamins of the B-group, PP and C. Investigation of PVC radioprotective action was carried out on the two groups of rats (n = 30) which received 1306 Bk/animal of Sr-85 or 343 Bk/animal of Cs-137 daily. One of the group of rats received every day also 300 mg of PVC. After 30 days level of Sr-85 was less by 56.6%, level of Cs = 137 was less by 27.6% in PVC-treated than in the control group (P < 0.05). Composite sorbent consisting of PVC and AC was slightly less effective in removing of Sr and Cs.

Topics: Animals; Cesium Radioisotopes; Drug Combinations; Drug Evaluation, Preclinical; Enterosorption; Female; Humans; Metals; Pectins; Poisoning; Radiation Injuries, Experimental; Rats; Strontium Radioisotopes; Vitamins

Topics: Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Azo Compounds; Body Weight; Cellulose; Cryptococcus; Diet; Female; Liver; Male; Medicago sativa; Pectins; Poisoning; Rats; Seeds; Time Factors; Vegetables

Topics: Adsorption; Aluminum Silicates; Antacids; Charcoal; Drug Evaluation, Preclinical; Gastric Juice; Gelatin; Gels; Ion Exchange Resins; Paraquat; Pectins; Poisoning; Soil

Topics: Animals; Anti-Inflammatory Agents; Bradykinin; Carrageenan; Cellulose; Dextrans; Edema; Guinea Pigs; Hindlimb; Ileum; Injections, Intraperitoneal; Injections, Intravenous; Kinins; Male; Pectins; Poisoning; Polyethylenes; Polysaccharides; Polyvinyls; Rats; Snakes; Sulfates; Time Factors; Venoms