pectins and Pancreatitis

Protecting the intestinal mucosa from being destroyed helps reduce the inflammation caused by acute pancreatitis (AP). In this study, whether okra pectin (OP) could attenuate the inflammation of AP through protecting the intestinal barrier was investigated.. OP was obtained from crude okra pectin (COP) through the purification by DEAE cellulose 52 column. Supplementation with OP or COP in advance reduced the severity of AP, as revealed by lower serum amylase and lipase levels, abated pancreatic edema, attenuated myeloperoxidase activity and pancreas histology. OP or COP inhibited the production of pancreatic proinflammatory cytokines, including tumor necrosis factor-α and interleukin-6. In addition, the upregulation of AP-related proteins including ZO-1, occludin, the antibacterial peptide-defensin-1 (DEFB1) and cathelicidin-related antimicrobial peptide (CRAMP), as well as the histological examination of colon injuries, demonstrated that OP or COP provision could effectively maintain intestinal barrier function. Ultimately, dietary OP or COP supplementation could inhibit AP-induced intestinal inflammation. For the above, the effect of OP was better than COP.. Dietary OP supplementation could be considered as a preventive method that effectively interferes with intestinal damage and attenuates inflammatory responses trigged by AP. © 2020 Society of Chemical Industry.

Topics: Abelmoschus; Animals; Ceruletide; Cytokines; Fruit; Humans; Intestinal Mucosa; Male; Mice; Occludin; Pancreatitis; Pectins; Plant Extracts; Tumor Necrosis Factor-alpha; Zonula Occludens-1 Protein

Acute pancreatitis (AP) is a common clinical acute abdominal disease. The intestinal injury associated with AP will aggravate the condition retroactively. This study investigates whether the low-methoxyl pectin (LMP) isolated from lemon could attenuate AP and associated intestinal injury.. Experimental AP was induced in BALB/c mice by caerulien (CAE) hyperstimulation. Nutritional prophylactic group was pre-fed with 5% LMP supplemented forage 3 days before AP induction. We found that LMP supplementation attenuated the severity of AP as evidenced by reduced serum amylase and lipase levels, pancreatic edema and myeloperoxidase activity. The protective effect was also confirmed by histological examination of pancreatic damage. LMP suppressed the production of pancreatic proinflammatory cytokines including TNF-α, IL-1β, and IL-6. Moreover, LMP supplementation restored AP-associated disruption of intestinal barrier integrity as evidenced by upregulation of tight junction modulatory proteins occludin, zonula occludens (ZO)-1, antimicrobial peptides β-defensin-1 (DEFB1) and CRAMP as well as increase in SCFAs production. LMP supplemented mice with AP exhibited suppressed intestinal inflammation as shown by decreased ileal and colon cytokine production compared with CAE group.. Our results support dietary LMP supplementation as an effective nutritional intervention for AP and associated intestinal injury.

Topics: Amylases; Animals; beta-Defensins; Ceruletide; Citrus; Lipase; Male; Mice; Mice, Inbred BALB C; Pancreatitis; Pectins; Tight Junctions

Topics: Adult; Aged; Aluminum Hydroxide; Drug Combinations; Drug Evaluation; Female; Gastric Juice; Gastrointestinal Hemorrhage; Hepatitis; Humans; Intubation, Gastrointestinal; Liver Cirrhosis; Male; Middle Aged; Pancreatitis; Pectins; Peritoneal Neoplasms; Thymine

Topics: Amylases; Anthraquinones; Colorimetry; Densitometry; Humans; Hydrogen-Ion Concentration; Kinetics; Methods; Pancreatitis; Pectins; Statistics as Topic; Sulfonic Acids