pectins and Hemorrhage

Topics: alpha-2-Antiplasmin; Aminocaproates; Aminocaproic Acid; Antifibrinolytic Agents; Asthma; Blood Platelets; Cholesterol; Fibrinolysis; Hemophilia A; Hemorrhage; Kidney Diseases; Liver Cirrhosis; Pectins; Pharmacology; Serotonin; Trypsin

Even though it is a common occurrence in practice, maintaining haemostasis can sometimes become a challenging issue in case of trauma, perioperative period, coagulation disorders, cancers, etc. Hemostatic materials are extensively used to assist in the cessation of bleeding. However, the definition of efficiency of haemostasis varies between intended procedures. This paper explores the feasibility of incorporating agents to increase the efficiency of local haemostasis. Pectin or β -D galacto hexopyranuronic acid/β Gal A, a structural polysaccharide widely present in terrestrial plants having an intrinsic hemostatic potential, is blended with gelatin and is explored in modulating passive haemostasis. The sponges are physico chemically characterized, and their hemostatic efficiency is evaluated in vitro using various assays. Biocompatibility evaluation is done by in vitro cytotoxicity assay. The results suggest that this biopolymer combination is a promising candidate for hemostatic control.

Topics: Gelatin; Hemorrhage; Hemostasis; Hemostatics; Humans; Pectins

New water-soluble pectin complexes with Ca(2+), Mg(2+), Co(2+), Cu(2+), Fe(2+), Mn(2+), Zn(2+) on the basis of pectin biopolymer have been synthesized and successfully tested on white rats. For a starting, we have obtained a sodium pectate to enhance solubility of target complexes as a whole. Shortly afterwards, running the reaction of ligand exchange of Nа(+) ions with corresponding s-, d- metal cations we were able to synthesize new pectin complexes. The ranges of s-, d-metals salts concentrations were detected experimentally, in which the selective formation of water-soluble complexes occurred. Antianemic effect of new pectin complexes with Na, Fe and Na, Ca, Fe was investigated on white rats with posthemorrhagic anemia. Under the effect of complexes, the improvement of animals and prevention of erythropoiesis disorders were observed. Antianemic effect of the complexes manifested itself in the doses equivalent to 25% or 50% of the iron daily rate, recommended in the treatment of iron-deficiency anemia with the drugs based on iron sulphate.

Topics: Anemia; Animals; Calcium; Hemorrhage; Iron; Organometallic Compounds; Pectins; Rats; Sodium

Evaluated were the anticoagulant and antithrombotic activities, and bleeding effect of two chemically sulfated polysaccharides, obtained from citric pectin, with different average molar masses. Both low-molecular-weight (Pec-LWS, 3,600 g/mol) and high-molecular-weight sulfated pectins (Pec-HWS, 12,000 g/mol) had essentially the same structure, consisting of a (1-->4)-linked alpha-D-GalpA chain with almost all its HO-2 and HO-3 groups substituted by sulfate. Both polysaccharides had anticoagulant activity in vitro, although Pec-HWS was a more potent antithrombotic agent in vivo, giving rise to total inhibition of venous thrombosis at a dose of 3.5 mg/kg body weight. Surprisingly, in contrast with heparin, Pec-HWS and Pec-LWS are able to directly inhibit alpha-thrombin and factor Xa by a mechanism independent of antithrombin (AT) and/or heparin co-factor II (HCII). Moreover, Pec-HWS provided a lower risk of bleeding than heparin at a dose of 100% effectiveness against venous thrombosis, indicating it to be a promising antithrombotic agent.

Topics: Animals; Anticoagulants; Blood Coagulation; Citrus sinensis; Disease Models, Animal; Dose-Response Relationship, Drug; Factor Xa Inhibitors; Female; Fibrinolytic Agents; Hemorrhage; Humans; Male; Molecular Weight; Pectins; Platelet Aggregation; Rats; Rats, Wistar; Structure-Activity Relationship; Sulfates; Thrombin; Venous Thrombosis

Topics: Hemophilia A; Hemorrhage; Hemostatics; Humans; Law Enforcement; Medicine; Pectins

Topics: Animals; Blood Volume; Hemorrhage; Hemostasis; Pectins; Rats

Topics: Ascorbic Acid; Hemorrhage; Humans; Pectins; Solutions; Syndrome; Vitamins