pectins and Fatty-Liver

Accumulating evidence has shown that gut microbiota and its metabolites have important significance in the etiology of obesity and related disorders. Prebiotics prevent and alleviate obesity by modulating the gut microbiota. However, how pectin oligosaccharides (POS) derived from pectin degradation affect gut microbiota and obesity remains unclear. To investigate the potential anti-obesity effects of POS, mice were fed a high-fat diet (HFD) for 12 weeks and a POS supplement with drinking water during the last 8 weeks. The outcomes demonstrated that POS supplementation in HFD-fed mice decreased body weight (

Topics: Animals; Diet, High-Fat; Fatty Liver; Gastrointestinal Microbiome; Mice; Mice, Inbred C57BL; Obesity; Oligosaccharides; Pectins

Pectin, a soluble fiber, improves non-alcoholic fatty-liver disease (NAFLD), but its mechanisms are unclear. We aimed to investigate the role of pectin-induced changes in intestinal microbiota (IM) in NAFLD. We recovered the IM from mice fed a high-fat diet, treated or not with pectin, to perform a fecal microbiota transfer (FMT). Mice fed a high-fat diet, which induces NAFLD, were treated with pectin or received a fecal microbiota transfer (FMT) from mice treated with pectin before (preventive FMT) or after (curative FMT) being fed a high-fat diet. Pectin prevented the development of NAFLD, induced browning of adipose tissue, and modified the IM without increasing the abundance of proteobacteria. Preventive FMT also induced browning of white adipose tissue but did not improve liver steatosis, in contrast to curative FMT, which induced an improvement in steatosis. This was associated with an increase in the concentration of short-chain fatty acids (SCFAs), in contrast to preventive FMT, which induced an increase in the concentration of branched SCFAs. Overall, we show that the effect of pectin may be partially mediated by gut bacteria.

Topics: Adipose Tissue, White; Animals; Diet, High-Fat; Fatty Liver; Fecal Microbiota Transplantation; Gastrointestinal Microbiome; Male; Mice, Inbred C57BL; Mice, Obese; Pectins

The hypolipidaemic and antisteatotic effects of the Konnyaku Powder (KP) have been reported before. In order to evaluate further the antisteatotic role played by KP, the effects of KP on the levels of liver lipid and on the hepatic histopathology and morphometry in comparison with those of pectin, algin and agar were studied. Sixty Wistar strain rats were divided into 6 groups: a normal diet group, a high cholesterol diet group (HC), and 4 test groups, in which the animals were fed on a diet similar to that of the high cholesterol diet group with addition of KP, pectin, algin or agar at a dosage of 5%, respectively. All the animals were killed at the end of the diet treatment for 9 weeks. The results showed that relative liver weights were lower in four experimental groups than in the HC group. The levels of total and free cholesterol, and triglyceride in the liver were lower in KP group than in HC group and in the other three experimental groups. Hepatic histopathology and morphometric examination indicated that antisteatotic effects of KP appeared to be much more significant than those of the other fibers.

Topics: Agar; Animals; Cholesterol, Dietary; Dietary Fiber; Fatty Liver; Liver; Male; Mannans; Pectins; Rats; Rats, Inbred Strains

Topics: Administration, Oral; Animal Feed; Animals; Cholelithiasis; Cholesterol; Cholesterol, Dietary; Cholestyramine Resin; Citrus; Dietary Fats; Fatty Liver; Female; Liver; Male; Pectins; Rabbits

Topics: Alcohols; Animals; Ascorbic Acid; Cholelithiasis; Cholesterol; Cholesterol, Dietary; Cholic Acids; Fatty Liver; Female; Guanidines; Male; Mice; Neomycin; Pectins; Phosphatidylcholines; Sulfonamides