pectins and Dehydration

Topics: Acidosis; Anthelmintics; Anti-Bacterial Agents; Candidiasis; Child; Dehydration; Diarrhea; Dysentery, Amebic; Dysentery, Bacillary; Escherichia coli Infections; Giardiasis; Humans; Intestinal Diseases, Parasitic; Kaolin; Lactose; Parasympatholytics; Pectins; Salmonella Infections

The addition of potato to wheat flour extends the nutritional values of bread. However, the adverse effects mediated by high dietary fiber in potato flour could affect the formation of gluten matrix. The water dynamics and distribution determined by the Low field nuclear magnetic resonance (LF-NMR) demonstrated a competitive water binding of dietary fiber, resulting in the partial dehydration and conformational changes of gluten protein complexes. Besides, the microstructure of the dough characterized by Scanning electron microscope (SEM) suggested that the insoluble cellulose could block the continuity of gluten from the spatial position, thereby negative affecting the mechanical properties of the dough. In our study, addition of cellulase and/or pectinase apparently mitigated the gluten aggregation and dehydration, contributing to the formation and the continuity of the three-dimensional gluten network. As a consequence, the specific volume of the bread was increased by 40.2%, and the hardness was reduced by 64.48%.

Topics: Bread; Cellulose; Dehydration; Dietary Fiber; Flour; Glutens; Pectins; Solanum tuberosum; Triticum; Water

Sewage treatment plants in Algeria produce huge quantities of sludge expressed in tons annually. This sludge produced is unfortunately contaminated because of the use of synthetic polyelectrolytes. Recently several kinds of research have been carried out on natural flocculants for sludge conditioning, because of several advantages they present such as their renewable source and their non-toxicity. This work aims to evaluate the potential use of protonated pectin extracted from orange waste of N'GAOUS juice factory as an eco-friendly flocculant in the chemical conditioning of sludge. Protonated pectin effectiveness was compared with synthetic cationic anionic and ionic polyelectrolytes (SUPERFLOC 8396, AF400, NF102). In this context, raw sludge samples from Bouira WWTP were tested. Specific resistance to filtration (SRF), cakes dry solid content were analyzed to determine filterability, dewatering capacity of conditioned sludge, and the optimum dose of each conditioner. So that our goal was to obtain greater dryness, which is the case with the addition of protonated pectin and even the addition of Superfloc, which allowed us to obtain dryness of 33.01% and 29.19%, respectively, for the same doses that gave the lowest SRF. Based on the results found and the analysis of the specific resistance to filtration (SRF) and the dryness, and compared with the values observed for the dewatered sludge by the method used in the Bouira WWTP. Band filters (18-22%) and raw sludge (4.8-5.7%).

Topics: Biomass; Biopolymers; Dehydration; Filtration; Flocculation; Humans; Pectins; Polyelectrolytes; Sewage; Waste Disposal, Fluid; Water

Plant cell walls undergo multiple cycles of dehydration and rehydration during their life. Calcium crosslinked low methoxy pectin is a major constituent of plant cell walls. Understanding the dehydration-rehydration behavior of pectin gels may shed light on the water transport and mechanics of plant cells. In this work, we report the contributions of the microstructure to the mechanics of pectin-Ca gels subjected to different extents of dehydration and subsequent rehydration. This is investigated using a pectin gel composition that forms 'egg-box bundles', a characteristic feature of the microstructure of low methoxy pectin-Ca gels. Large amplitude oscillatory shear (LAOS) rheology along with small angle neutron scattering and near infrared (NIR) spectroscopy on pectin gels is used to elucidate the mechanical and microstructural changes during dehydration-rehydration cycles. As the extent of dehydration increases, the reswelling ability, strain-stiffening behavior and yield strain decrease. These effects are more prominent at faster rates of dehydration and are not completely reversible upon rehydration to the initial undried state. Microstructural changes due to the aggregation of egg-box bundles and single chains and the associated changes in the water configurations lead to these irreversible changes.

Topics: Calcium; Cell Wall; Dehydration; Gels; Humans; Pectins; Plant Cells; Rheology; Water

Craterostigma plantagineum belongs to the desiccation-tolerant angiosperm plants. Upon dehydration, leaves fold and the cells shrink which is reversed during rehydration. To understand this process changes in cell wall pectin composition, and the role of the apoplastic glycine-rich protein 1 (CpGRP1) were analysed. Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections were analysed using scanning electron microscopy. Pectin composition in different cell wall fractions was analysed with monoclonal antibodies against homogalacturonan, rhamnogalacturonan I, rhamnogalacturonan II and hemicellulose epitopes. Our data demonstrate changes in pectin composition during dehydration/rehydration which is suggested to affect cell wall properties. Homogalacturonan was less methylesterified upon desiccation and changes were also demonstrated in the detection of rhamnogalacturonan I, rhamnogalacturonan II and hemicelluloses. CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves. CpGRP1 can also bind phosphatidic acid (PA) and cardiolipin. The binding of CpGRP1 to pectin appears to be dependent on the pectin methylesterification status and it has a higher affinity to pectin than its binding partner CpWAK1. It is hypothesised that changes in pectin composition are sensed by the CpGRP1-CpWAK1 complex therefore leading to the activation of dehydration-related responses and leaf folding. PA might participate in the modulation of CpGRP1 activity.

Topics: Arginine; Cell Wall; Craterostigma; Dehydration; Pectins; Phosphatidic Acids; Plant Leaves; Plant Proteins

Date palm (Phoenix dactylifera) is an important crop providing a valuable nutrition source for people in many countries including the Middle East and North Africa. In recent years, the amount of rain in North Africa and especially in the Tunisian palm grove areas has dropped significantly. We investigated the growth and cell wall remodelling of fruits harvested at three key development stages from trees grown with or without water supply. During development, cell wall solubilization and remodelling was characterized by a decrease of the degree of methylesterification of pectin, an important loss of galactose content and a reduction of the branching of xylan by arabinose in irrigated condition. Water deficit had a profound effect on fruit size, pulp content, cell wall composition and remodelling. Loss of galactose content was not as important, arabinose content was significantly higher in the pectin-enriched extracts from non-irrigated condition, and the levels of methylesterification of pectin and O-acetylation of xyloglucan were lower than in irrigated condition. The lower levels of hydrophobic groups (methylester and O-acetyl) and the less intensive degradation of the hydrophilic galactan, arabinan and arabinogalactan in the cell wall may be implicated in maintaining the hydration status of the cells under water deficit.

Topics: Acetylation; Arecaceae; Cell Wall; Dehydration; Esterification; Fruit; Galactans; Galactose; Glucans; Hydrophobic and Hydrophilic Interactions; Pectins; Polysaccharides; Solubility; Water; Xylans

The structure of composites produced by the bacterium Acetobacter xylinus have been studied in their natural, hydrated, state. Small-angle X-ray diffraction and environmental scanning electron microscopy has shown that the ribbons have a width of 500 A and contain smaller semi-crystalline cellulose microfibrils with an essentially rectangular cross-section of approximately 10 x 160 A(2). Incubation of Acetobacter in xyloglucan or pectin results in no changes in the size of either the microfibrils or the ribbons. Changes in the cellulose crystals are seen upon dehydration of the material, resulting in either a reduction in crystal size or an increase in crystal disorder.

Topics: Acetobacter; Cellulose; Dehydration; Glucans; Microscopy, Electron, Scanning; Models, Statistical; Pectins; Polysaccharides; Scattering, Radiation; Water; X-Ray Diffraction; Xylans