pectins and Alzheimer-Disease

Pectin is a class of complex polysaccharides and recognized for its potential bioactivities. In this study, we showed that a pectic polysaccharide, LFA03-a, was extracted from Lonicera japonica Thunb. flowers and purified with DEAE-cellulose and Sephacryl S-100HR. LFA03-a was composed of rhamnose, arabinose, galactose and galacturonic acid in the molar ratio of 18.1:25.3:36.8:19.5. Its structure was determined to possess a rhamnogalacturonan I (RG-I) backbone consisting of α-l-1,2-Rhap and α-d-1,4-GalAp disaccharide repeating unit, substituted at O-4 of l-rhamnose. The side chain was involved with β-d-1,4-Galp, β-d-1,3-Galp, β-d-1,3,6-Galp and branched α-l-1,5-Araf. Fluorescence spectroscopic analysis with thioflavine T (ThT) and atomic force microscopy (AFM) results showed that LFA03-a inhibited Aβ

Topics: Alzheimer Disease; Amyloid beta-Peptides; Animals; Arabinose; Flowers; Galactose; Hexuronic Acids; Humans; Lonicera; Microscopy, Atomic Force; Molecular Structure; PC12 Cells; Pectins; Polysaccharides; Protein Aggregation, Pathological; Rats; Rhamnose; Spectrometry, Fluorescence