pantetheine and Tuberculosis

Phosphopantetheinyl hydrolase, PptH (Rv2795c), is a recently discovered enzyme from Mycobacterium tuberculosis that removes 4'-phosphopantetheine (Ppt) from holo-carrier proteins (CPs) and thereby opposes the action of phosphopantetheinyl transferases (PPTases). PptH is the first structurally characterized enzyme of the phosphopantetheinyl hydrolase family. However, conditions for optimal activity of PptH have not been defined, and only one substrate has been identified. Here, we provide biochemical characterization of PptH and demonstrate that the enzyme hydrolyzes Ppt

Topics: Animals; Bacterial Proteins; Cell Wall; Female; Humans; Lipids; Mice; Mice, Inbred C57BL; Mycobacterium tuberculosis; Pantetheine; Phosphoric Diester Hydrolases; Protein Processing, Post-Translational; Tuberculosis; Virulence