oxytetracycline--anhydrous and Skin-Ulcer

A new virus disease that displays dermal ulceration and high mortality has been occurring since 1996 in color carp Cyprinus carpio reared in warm water in Japan. In histological examinations, initial erosive lesions displayed necrosis, hemorrhage and fibrin deposition in the dermal loose connective tissue and were accompanied by the partial destruction of the epidermis. Developed ulcerative lesions involved the lateral musculature with bacterial invasions. In visceral organs, necrotic cells were observed in the hematopoietic tissue, the spleen and the intestinal tissues as well as in cardiac muscle fibers which showed no signs of bacterial invasion. Electron microscopy revealed corona-like virus particles in these necrotic cells. The necrotic cells of the hematopoietic tissue and the spleen were accompanied by the formation of tubular structures and crystalline inclusions. The putative virus was isolated and cultured in epithelioma papillosum cyprini (EPC) cells. Carp experimentally inoculated with the cultured virus showed virus transmission, and the same pathological signs of the disease and mortalities as in natural infections.

Topics: Animals; Anti-Bacterial Agents; Carps; Coronaviridae Infections; Coronavirus; Cytopathogenic Effect, Viral; Disease Outbreaks; Fish Diseases; Histocytochemistry; Japan; Microscopy, Electron; Muscle, Smooth, Vascular; Oxytetracycline; Skin; Skin Ulcer; Spleen; Viremia; Virion

Topics: Abscess; Adult; Chronic Disease; Drug Combinations; Drug Therapy, Combination; Erythromycin; Female; Folliculitis; Furunculosis; Humans; Male; Middle Aged; Oxytetracycline; Pyoderma; Skin Ulcer; Staphylococcal Infections; Streptococcal Infections

The clinically used topical antibiotic ointments, gentamicin 0.1%, oxytetracycline 3% w/polymyxin 0.1% and chloramphenicol 2% and an experimental preparation, 10-undecen-1-yl thiopseudourea iodide (AHR-1911) were studied for anti-infective action applied externally on the skin of mice inoculated subcutaneously with S. aureus and E. coli. In both infections statistically significant difference was encountered between curative and "clinical" healing rate in the case of the less effective preparations and/or dosages. The method appears suitable to establish and to compare the in vivo activity of ointments. This is not directly related to that of the aqueous drug solutions. AHR-1911, a powerful inhibitor of nucleic acid and protein synthesis, showed a bimodal action, with maximal effectiveness at 0.12-0.25% concentration, due presumably to the anti-inflammatory effect of higher concentrations. Direct evidence for the absorption of the drug from the application site was obtained using 14C labeled AHR-1911.

Topics: Abscess; Administration, Topical; Animals; Drug Combinations; Drug Evaluation; Escherichia coli Infections; Gentamicins; Isothiuronium; Mice; Ointments; Oxytetracycline; Polymyxins; Skin Ulcer; Staphylococcal Infections; Thiourea

Topics: Adolescent; Ascorbic Acid; Ethacridine; Female; gamma-Globulins; Humans; Infant; Male; Oxytetracycline; Skin Ulcer; Smallpox Vaccine; Thiamine; Vaccination; Vaccinia

Topics: Adolescent; Adult; Aged; Chlortetracycline; Chronic Disease; Climate; Humans; Middle Aged; Oxytetracycline; Penicillins; Phenols; Silver Nitrate; Skin Ulcer; Sulfonamides; Vitamins

Topics: Adult; Cysteine; Humans; Iron; Male; Methyltestosterone; Oxytetracycline; Skin Ulcer; Sulfates; Vitamins; Werner Syndrome

Topics: Breast Neoplasms; Humans; Hydrocortisone; Oxytetracycline; Skin Ulcer