oxytetracycline--anhydrous and Necrosis

To comparatively investigate the effects melatonin and oxytetracycline as antioxidants on autologous intraperitoneal ovary transplantation in rats.. 28 adult female Wistar rats with regular estrous cycles were randomly divided into four groups. Group 1 (n=7) was the control group; Group 2 (n=7) bilateral oophorectomy and intraperitoneal transplantation group; and Groups 3 (n=7) and 4 (n=7) firstly received 20 mg/kg/IP melatonin and 10 mg/kg/IP oxytetracycline., respectively and fifteen minutes after that, they had bilateral oophorectomy and intraperitoneal transplantation. Ovarian tissue and blood malondialdehyde (MDA) levels, superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) levels were determined. Vaginal cytology and ovarian necrosis were histopathologically investigated. Kruskall Wallis analysis of variance was used in the statistical analysis of data.. Ovarian necrosis, tissue MDA and plasma MDA levels were significantly higher in Group 2 compared to the Groups 1, 3 and 4 (p<0.03). SOD and GSHPx levels were significantly lower in group 2 compared to the groups 1, 3 and 4 (p<0.03). Melatonin was found to be more effective on ovarian necrosis and tissue MDA level than the use of oxytetracycline following autologous intraperitoneal ovary transplantation (p<0.03). There was a significant positive correlation between tissue MDA level and ovarian necrosis and a very strong negative correlation between tissue MDA and SOD level (rs = +/-0.9, n=28, p=0.000).. Although both melatonin and oxytetracycline were found to be effective in autologous intraperitoneal pelvic ovary transplantation, melatonin is more effective against both the ovarian necrosis and tissue MDA level in rats.

Topics: Animals; Anti-Bacterial Agents; Antioxidants; Female; Graft Survival; Malondialdehyde; Melatonin; Necrosis; Ovary; Oxytetracycline; Peritoneal Cavity; Rats; Rats, Wistar

A number of techniques were employed to assess cell death induced in honeybee larvae midgut after per os inoculation of bacterium Paenibacillus larvae var. larvae, the causative agent of American foulbrood disease, and separately with acaricide Amitraz and antibiotic Oxytetracycline. In honeybee larvae exposed to Amitraz, which demonstrates both necrosis and apoptosis, cell death was found in 82% of midgut columnar and in 50% of regenerative epithelial cells, 24 h after treatment. Cell death reduced to 36% in the epithelial cells, 48 h after treatment. In Oxytetracycline-treated larvae, cell death was identified in 40% of midgut epithelial cells, 24 h after inoculation and increased to 55% over the next 24 h. In Paenibacillus -infected larvae, all midgut epithelial cells died. Using ApopTag (Oncor) to label the multiple DNA ends generated by DNA fragmentation showed programmed cell death in 49% of columnar midgut cells 24 h after Amitraz application. Cell death was reduced to 9% over the next 24 h. Our data indicate that cell death could be identified and quantified in situ, using TUNEL techniques. This study also shows that the acaricide Amitraz is a trigger for programmed cell death in the midgut epithelial cells of honeybee larvae, unlike Paenibacillus which induces necrosis only. The data show that immunohistochemical methods are useful for studying in situ tissue pathology, and indicate possibilities for monitoring the effects of infective and chemical environmental stressors on cell death in honeybee larvae tissue.

Topics: Adrenergic alpha-Agonists; Alkaline Phosphatase; Animals; Anti-Bacterial Agents; Apoptosis; Bacillus; Bees; Cell Nucleus; Cytoplasm; Digestive System; DNA Fragmentation; Epithelial Cells; Evaluation Studies as Topic; Humans; Immunohistochemistry; In Situ Nick-End Labeling; Larva; Necrosis; Oxytetracycline; Peroxidase; Toluidines; Tumor Cells, Cultured

Topics: Animals; Animals, Zoo; Artiodactyla; Female; Gram-Negative Bacteria; Mouth Mucosa; Necrosis; Oxytetracycline; Pasteurella; Pregnancy; Stomatitis

Severe necro-purulent lesions were induced in mice following parenteral inoculation of Sphaerophorus necrophorus. Gross and histological changes observed in the lung, liver, and foot pad of infected mice were similar to those occurring naturally in cattle. The lesions could be prevented, cured or significantly reduced by the administration of chemotherapeutic agents such as sulfonamides, potentiated sulfonamide and antibiotics. The application of this novel laboratory model infection in the primary evaluation of potential antibacterial agents is discussed.

Topics: Animals; Chlortetracycline; Foot; Fusobacterium Infections; Fusobacterium necrophorum; Injections; Injections, Subcutaneous; Leucomycins; Liver; Lung; Male; Mice; Microbial Sensitivity Tests; Necrosis; Neomycin; Oxytetracycline; Penicillins; Pyrimidines; Skin; Sulfadimethoxine; Sulfamethoxazole; Thorax

Topics: Cell Line; Chlortetracycline; Demeclocycline; Liver; Necrosis; Oxytetracycline; Technetium; Tetracycline

Topics: Animals; Drug Combinations; Injections, Intramuscular; Muscles; Necrosis; Oxytetracycline; Povidone; Skin; Sulfamethazine; Swine

Topics: Amniotic Fluid; Animals; Anti-Bacterial Agents; Female; Fetus; Guinea Pigs; Liver; Necrosis; Oxytetracycline; Penicillin G Benzathine; Penicillins; Placenta; Placenta Diseases; Pregnancy; Pregnancy, Animal; Stomach; Streptomycin; Uterus; Vibrio Infections

Topics: Adult; Aged; Female; Humans; Leg; Male; Methods; Middle Aged; Necrosis; Oxytetracycline; Postoperative Complications; Premedication; Veins; Wound Healing

Topics: Animals; Antibiotics, Antineoplastic; Antibody Formation; Arthus Reaction; Cortisone; Depression, Chemical; Flavonoids; Hyperemia; Immune Sera; Male; Mercaptopurine; Necrosis; Oxytetracycline; Precipitins; Rabbits; Time Factors

Topics: Animals; Cecum; Chlortetracycline; Colon, Sigmoid; Cricetinae; Diarrhea; Drug Hypersensitivity; Epithelium; Intestine, Large; Necrosis; Oxytetracycline; Tetracycline; Vascular Diseases

Topics: Acid Phosphatase; Aminopeptidases; Animals; Electron Transport Complex IV; Fluorescence; Histocytochemistry; Kidney; Male; Mice; Microscopy, Fluorescence; Necrosis; Oxytetracycline; Radiation Injuries, Experimental

Topics: Amylases; Animals; Aprotinin; Blood Glucose; Intestinal Obstruction; Intestine, Small; Necrosis; Oxytetracycline; Pancreas; Rats

Topics: Blood; gamma-Globulins; Glycoproteins; Hepatectomy; Hyperplasia; Hypertrophy; Kidney Diseases; Liver Diseases; Necrosis; Neoplasms; Neoplasms, Experimental; Oxytetracycline; Rats; Research; Wounds and Injuries

Topics: Administration, Topical; Alopecia; Animals; Anti-Bacterial Agents; Blister; Cortisone; Dermatologic Agents; Edema; Erythema; Eye; Framycetin; Guinea Pigs; Hydrocortisone; Mice; Necrosis; Neoplasms, Radiation-Induced; Oxytetracycline; Prednisolone; Radiation Injuries; Research; Skin

Topics: Animals; Femur Head; Femur Head Necrosis; Fluorescence; Injections; Injections, Intramuscular; Necrosis; Osteonecrosis; Oxytetracycline; Rabbits; Research; Ultraviolet Rays

Topics: Aerosols; Burns; Drug Combinations; Gangrene; Humans; Hydrocortisone; Leg Ulcer; Necrosis; Oxytetracycline; Wound Infection