oxytetracycline--anhydrous and Liver-Neoplasms

A study was made of the efficacy of trypan blue, acridine orange, tetracycline and oxytetracycline for detection of tumour cells injected into the blood stream of rats. The cells were identified in the mesenteric microvessels by intravital microscopy. Fluorescence of fluorochromized cells was observed in the blue-violet (lambda max = 400 nm) and ultra-violet (lambda max = 365 nm) irradiation of the fluorescent lamp and in the laser irradiation (lambda = 337 nm). The cells stained with acridine orange had a higher fluorescence intensity and a more distinct structure than those labelled with tetracyclines. Identification of cells with trypan blue was more difficult. The fluorescent method of determination is rather simple and permits to indentify tumour cells directly in the blood stream.

Topics: Acridines; Animals; Carcinoma, Hepatocellular; Liver Neoplasms; Microscopy, Fluorescence; Neoplasms, Experimental; Neoplastic Cells, Circulating; Oxytetracycline; Rats; Staining and Labeling; Tetracycline; Trypan Blue

Sprague-Dawley rats were given combinations of aminopyrine or oxytetracycline and sodium nitrite in drinking water. Of 30 animals receiving 0.1% (1,000 ppm) of aminopyrine and sodium nitrite for 30 weeks, 29 died with hemangioendothelial sarcomas of the liver. The same tumor caused death in 26 of 30 animals that received 0.025% (250 ppm) of both aminopyrine and sodium nitrite for 50 weeks. No animals in a control group of the same size that received 0.1% aminopyrine for 30 weeks developed this tumor, although one-half of them were still alive 2 years after the experiment was begun. After feeding a comparable dose (0.1%) of oxytetracycline and sodium nitrite for 60 weeks, liver tumors were present in 4 of 30 rats (3 hepatocellular tumors and 1 cholangioma). Since aminopyrine has been widely used for medicinal purposes in the human population, it is possible that many people have been exposed to a potent carcinogen (dimethylnitrosamine) by its formation in vivo. It is not certain whether the result of feeding oxytetracycline and sodium nitrite indicates significant carcinogenicity of this combination.

Topics: Adenoma, Bile Duct; Aminopyrine; Animals; Carcinogens; Carcinoma, Hepatocellular; Drug Combinations; Hemangioendothelioma; Liver Neoplasms; Neoplasms, Experimental; Nitrites; Oxytetracycline; Rats; Sodium; Time Factors

Topics: Albumins; gamma-Globulins; Hemangioendothelioma; Hemangiosarcoma; Humans; Infant; Liver Neoplasms; Oxytetracycline; Pathology; Radiotherapy; Surgical Procedures, Operative