oxytetracycline--anhydrous and Asthma

Topics: Asthma; Bronchitis; Clinical Trials as Topic; Expectorants; Humans; Oxytetracycline; Pulmonary Emphysema; Sputum

Oxytetracycline has been used in the treatment of acute and chronic bronchial inflammation and infectious asthma. However, its potential use for non-infectious asthma has not yet been studied. The objective of this study was to investigate the anti-inflammatory properties of oxytetracycline using a mouse asthma model. Female BALB/c mice, sensitized and challenged with ovalbumin. Naive CD4+ T cells from spleen were stimulated for 72 h with anti-CD3 (5 μg/ml) plus anti-CD28 (2.5 μg/ml) and differentiated into Th2 cells. IL-4, IL-5, IL-9, IL-13, and ovalbumin (OVA)-specific IgE production were measured by ELISA in BALF and cell supernatants. Histopathological evaluation was used to study the alterations in lung tissue. The mRNA levels of CCL5, CCL11, CCR1, and CCR3 were detected by real-time PCR. In addition, the protein levels of p-Akt, Akt, nuclear factor kappa B (NF-κB), IκBα and p-IκBα in lung tissue and cells were measured by western blot or immunofluorescence analysis. Oxytetracycline treatment caused a marked reduction in IL-4, IL-5, IL-13, immune cells, and the level of ovalbumin-specific IgE. Real-time PCR studies demonstrated that oxytetracycline can significantly reduce CCL5, CCL11 and their specific receptor CCR1 and CCR3. Histological studies demonstrated that oxytetracycline substantially inhibited ovalbumin-induced inflammatory cell infiltration in lung tissue and goblet cell hyperplasia in airway. Oxytetracycline inhibited the NF-κB activation via phosphorylation and degradation of IκBα both in vivo and in vitro. Furthermore, the increased phosphorylated Akt but not Akt protein levels in lung tissues after OVA inhalation were significantly reduced by the oral administration of oxytetracycline. These findings demonstrate an anti-inflammatory effect of oxytetracycline that might be mediated via reduction of inflammatory mediators and activation of transcription factors.

Topics: Animals; Anti-Asthmatic Agents; Asthma; Cytokines; Disease Models, Animal; Enzyme Activation; Female; Gene Expression Regulation; I-kappa B Proteins; Immunoglobulin E; Inflammation; Mice; Mice, Inbred BALB C; NF-kappa B; NF-kappaB-Inducing Kinase; Ovalbumin; Oxytetracycline; Phosphorylation; Protein Serine-Threonine Kinases; Proto-Oncogene Proteins c-akt; Respiratory System; Signal Transduction

Topics: Asthma; Chronic Disease; Doxycycline; Humans; Oxytetracycline; Respiratory Tract Infections

Six asthmatic patients whose airway obstruction improved significantly during a trial of prednisone have been studied in detail. Serial measurements of forced expiratory volume in 1 second (FEV1) were made at specific times each day before and after isoprenaline, for 8 days. Peak response was at 5 minutes in the majority of tests. In contrast to previous studies no clear pattern of response emerged in relation either to type of asthma, length of history or initial FEV1.

Topics: Adult; Airway Resistance; Albuterol; Asthma; Female; Forced Expiratory Volume; Humans; Isoproterenol; Male; Metaproterenol; Middle Aged; Oxytetracycline; Peak Expiratory Flow Rate; Prednisone; Time Factors

Topics: Aged; Asthma; Female; Humans; Oxytetracycline; Strongyloides; Strongyloidiasis; Vitamin B Complex

Topics: Acetylcysteine; Aniline Compounds; Asthma; Bronchitis; Chronic Disease; Chymotrypsin; Expectorants; Humans; Oxytetracycline; Sputum; Toluene

Topics: Asthma; Bronchitis; Bronchodilator Agents; Chronic Disease; Expectorants; Humans; Oxytetracycline; Quaternary Ammonium Compounds

Topics: Adult; Anti-Bacterial Agents; Asthma; Bacillus cereus; Female; Humans; Neomycin; Oleandomycin; Oxytetracycline; Pleurisy; Prednisone

Topics: Adult; Aged; Amines; Asthma; Bronchitis; Emphysema; Humans; Middle Aged; Oxytetracycline

Topics: Adult; Aerosols; Aged; Asthma; Bronchitis; Female; Humans; Male; Middle Aged; Oxytetracycline; Pulmonary Emphysema

Topics: Anti-Bacterial Agents; Antitubercular Agents; Asthma; Bronchiectasis; Bronchitis; Chloramphenicol; Chlortetracycline; Chymotrypsin; Empyema; Humans; Hydrocortisone; Kanamycin; Oxytetracycline; Penicillins; Peptide Hydrolases; Pleurisy; Pneumonia; Pulmonary Emphysema; Respiratory System; Respiratory Tract Diseases; Tuberculosis; Tuberculosis, Pulmonary

Topics: Asthma; Biomedical Research; Bronchitis; Bronchopneumonia; Child; Chlortetracycline; Double-Blind Method; Drug Therapy; Escherichia coli; Haemophilus influenzae; Humans; Nasopharynx; Oxytetracycline; Pneumonia; Preventive Medicine; Proteus; Pseudomonas; Radiography, Thoracic; Staphylococcus; Streptococcus pneumoniae; Toxicology

Topics: Anti-Bacterial Agents; Antibiotics, Antitubercular; Asthma; Bronchitis; Emphysema; Humans; Muscle Spasticity; Oxytetracycline; Prednisolone; Pulmonary Emphysema

Topics: Asthma; Communicable Diseases; Oxytetracycline

Topics: Adrenocorticotropic Hormone; Anti-Bacterial Agents; Asthma; Communicable Diseases; Disease Management; Oxytetracycline