oxytetracycline--anhydrous and Adenocarcinoma

A prospective randomized study on the effects of Enterobiotic (oxytetracycline + neomycin) given per os to patients operated upon electively and curatively for carcinoma coli and recti is presented. 75 patients were treated and 66 were controls. A highly significant reduction of peroperative total and Gram-negative bacterial growth from the bowel was registered and a probably significant reduction of anaerobic bacterial growth. In the whole material a highly significant lower incidence of wound sepsis and intra-abdominal infectious complications occurred in the treated group, compared with the control group. This applies also to the resection material, while the difference is of probable significance in the excision group. 13 of 66 patients died, 8 of septic complications, all in the non-treated group. There was no mortality in the pretreated group. The difference between the noninfected and the infected patient groups was significant as regards the preoperative intraluminal total growth of bacteria and the growth of Gram-negative bacteria. The subsequent non-infected patients show a significantly increased number of cultures not showing any growth of bacteria. No negative side effects of Enterobiotic therapy were noted.

Topics: Adenocarcinoma; Aged; Bacterial Infections; Bacteriological Techniques; Colonic Neoplasms; Drug Combinations; Feces; Female; Humans; Intestines; Intraoperative Care; Male; Neomycin; Oxytetracycline; Postoperative Care; Prospective Studies; Random Allocation; Rectal Neoplasms; Surgical Wound Infection

In the available literature some evidence has been shown, implying that antibiotic prophylaxis in connection with colorectal surgery might increase the frequency of local recurrences of the carcinoma. 134 patients undergoing elective curative surgery of the large bowel have been followed for 20-52 months. 66 patients had been pretreated with Enterobiotic; 68 patients were controls. In our study, we found no difference between the pretreated and non-pretreated group concerning the frequency of anastomotic suture line recurrences or other types of local recurrences in the operation field.

Topics: Adenocarcinoma; Aged; Bacterial Infections; Clinical Trials as Topic; Colon; Colonic Neoplasms; Follow-Up Studies; Humans; Middle Aged; Neomycin; Neoplasm Recurrence, Local; Neoplasm Seeding; Oxytetracycline; Preoperative Care; Random Allocation; Rectal Neoplasms; Risk; Surgical Wound Infection; Tablets

Topics: Adenocarcinoma; Adult; Bile Duct Neoplasms; Clinical Trials as Topic; Drug Combinations; Esophagus; Female; Fluorouracil; Gastrointestinal Neoplasms; Humans; Male; Mechlorethamine; Middle Aged; Oxytetracycline; Pancreas; Pancreatic Neoplasms; Pharmaceutical Vehicles; Rectum; Retroperitoneal Neoplasms

Normal prostate tissue contains high levels of citrate. In the presence of prostate cancer, the citrate level is diminished. In this paper we show that it is possible to use europium-oxytetracycline complex as a citrate fluorescent probe and consequently as a prostate cancer probe. We analyzed normal nude male mice urine and urine from nude male mice in which prostate cancer was induced by intraprostatic inoculation of DU145 cells. The urine samples were collected from the animals at the 7th, 14th, 21st, and 35th days after the surgery procedures. The intensity of europium emission at 615 nm in europium-oxytetracycline complex in the presence of citrate increases linearly. The citrate concentrations were determined from a calculated calibration curve. A concentration decrease in malignant prostate urine from the normal (PBS group) urine value from ~8.0 mM to ~2.4 mM (tumor group at 35th day) was found. The obtained results indicated that europium-oxytetracycline provides a significant biomarker for prostate cancer detection with a direct, accurate, noninvasive, and non-enzymatic method for measurement of citrate in biological fluids.

Topics: Adenocarcinoma; Animals; Calibration; Cell Line, Tumor; Citrates; Coordination Complexes; Disease Progression; Early Diagnosis; Fluorescent Dyes; Humans; Male; Mice; Mice, Nude; Osmolar Concentration; Oxytetracycline; Prostatic Neoplasms; Spectrometry, Fluorescence; Urinalysis

Pharmaceuticals in the environment are of growing concern for their potential consequences on human and ecosystem health. Alterations in the endocrine system in humans or wildlife are of special interest because these alterations could eventually lead to changes in reproductive fitness. Using the H295R cell line, the potential endocrine disrupting effects of six pharmaceuticals including diclofenac, erythromycin, sulfamethazine, sulfathiazole, oxytetracycline, and chlortetracycline were investigated. After exposure to each target pharmaceutical for 48 h, production of 17beta-estradiol (E2) and testosterone (T), aromatase (CYP19) enzyme activity, or expression of steroidogenic genes were measured. Concentrations of E2 in blood plasma were determined in male Japanese medaka fish after 14 d exposure to sulfathiazole, oxytetracycline, or chlortetracycline. Among the pharmaceuticals studied, sulfathiazole, oxytetracycline and chlortetracycline all significantly affected E2 production by H295R cells. This mechanism of the effect was enhanced aromatase activity and up-regulation of mRNAs for CYP17, CYP19, and 3betaHSD, all of which are important components of steroidogenic pathways. Sulfathiazole was the most potent compound affecting steroidogenesis in H295R cells, followed by chlortetracycline and oxytetracycline. Sulfathiazole significantly increased aromatase activity at 0.2 mg/l. In medaka fish, concentrations of E2 in plasma increased significantly during 14-d exposure to 50 or 500 mg/l sulfathiazole, or 40 mg/l chlortetracycline. Based on the results of this study, certain pharmaceuticals could affect steroidogenic pathway and alter sex hormone balance. Concentrations of the pharmaceuticals studied that have been reported to occur in rivers of Korea are much less than the thresholds for effects on the endpoints studied here. Thus, it is unlikely that these pharmaceuticals are causing adverse effects on fish in those rivers.

Topics: Adenocarcinoma; Animals; Cell Line, Tumor; Chlortetracycline; Gonadal Steroid Hormones; Humans; Male; Oryzias; Oxytetracycline; Polymerase Chain Reaction; Sulfathiazole; Sulfathiazoles; Water Pollutants, Chemical