oxepins and Hemolysis

A review of new Zoapatle publications was made, including five chemical compounds characterized recently. Relative potency of Kaurenoic acid, Kauradienoic acid (its mixture), Zoapatanol and Montanol was estimated in an in vitro guinea pig uterine assay. Kaurene compounds were several times more potent than Montanol and Zoapatanol as uteroactive substances. Zoapatle aqueous crude extract made from Montanoa frutescens had a strong in vitro uterine potency and unique in vivo characteristics in several biological systems, described in detail in the following five publications.

Topics: Abortifacient Agents, Nonsteroidal; Abortion, Induced; Animals; Cardiovascular System; Cervix Uteri; Chemistry, Pharmaceutical; Contraceptive Agents, Female; Corpus Luteum; Cricetinae; Dogs; Endometrium; Female; Guinea Pigs; Haplorhini; Hemolysis; Humans; Labor, Induced; Male; Mice; Montanoa; Oxepins; Plant Extracts; Plants, Medicinal; Pregnancy; Rats; Receptors, Cell Surface; Sperm Immobilizing Agents; Spermatozoa; Structure-Activity Relationship; Uterine Contraction; Vasoconstrictor Agents

The effect of zoapatle aqueous crude extract (ZACE) was further studied and partially characterized upon human and rabbit spermatozoa. ZACE prepared from Montanoa tomentosa s.s.p tomentosa did not influence sperm motility or viability in a wide range of ZACE concentrations tested; on the other hand, ZACE prepared from Montanoa frutescens had immediate and constant inhibitory effect upon motility and decreased cell viability. Red cell lysis was readily observed with ZACE-frutescens, but not with ZACE-tomentosa. The effect of time on ZACE-frutescens potency for inducing red cell lysis was observed.

Topics: Animals; Biological Assay; Cell Survival; Erythrocytes; Hemolysis; Humans; Male; Montanoa; Oxepins; Plant Extracts; Plants, Medicinal; Rabbits; Sperm Immobilizing Agents; Sperm Motility; Spermatozoa