ovalbumin and Seizures

Allergic rhinitis is a systemic disease with high prevalence, which some of its neuropsychological problems have been reported. The primary pathophysiology and mechanism of the neuropsychological dysfunction of AR patients have not been described yet, so here we subjected an animal model of AR to identify any behavioral or seizure threshold changes and to assess the pathophysiology of the disease.. Eighty male BALB/C mice were randomly divided into the allergic rhinitis group and controls. Allergic rhinitis was induced in the first group by administering OVA and aluminum hydroxide intraperitoneally and then nasal injection of OVA for 14 consecutive days. Both groups were subjected to different tests for assessing depressive-like behavior, anxiety, spatial and contextual memory, and learning and seizure threshold. Hippocampus and plasma samples of mice were subjected for analyzing cytokines and immune modulators and for pathology and immunohistochemistry evaluation.. The depressive and anxiety-like behavior were increased in AR, and the spatial learning and memory were disturbed in the AR group. Also, AR mice had lower seizure thresholds compared to controls. Lab data suggested that TLR4, NF-κB, IL-1β, and TNFα expressions were increased in the AR hippocampus as well as their plasma proinflammatory cytokines. Likewise, demyelination, cell death, and M1 macrophage aggregation were increased in the AR hippocampus.. Behavioral and cognitive problems should be taken seriously in patients with AR or other atopic diseases, and more investigating is required to clear the pathophysiology behind it and its treatment.

Topics: Animals; Cytokines; Disease Models, Animal; Hippocampus; Humans; Immunoglobulin E; Male; Mice; Mice, Inbred BALB C; Nasal Mucosa; Neuroinflammatory Diseases; NF-kappa B; Ovalbumin; Rhinitis, Allergic; Seizures; Signal Transduction; Toll-Like Receptor 4

Prior reports show that nebulized lidocaine might be an effective treatment for asthma.. We sought to determine the anti-inflammatory and spasmolytic effects of lidocaine and its analogue, JMF2-1, which we have synthesized for reduced local anesthetic activity.. Blockade of Na(+) currents was assayed in cultured GH(3) cells by using the patch-clamp technique, whereas anesthesia was assessed in a cutaneous pinching test in rats. Lidocaine and its analogue were nebulized into sensitized rats for evaluation of their effectiveness on airways spasm and inflammation induced by methacholine and allergen, respectively. Tissue histamine release and tracheal spasm triggered by allergen challenge in the absence and presence of these treatments were also examined in vitro.. The 50% inhibitory concentration values for blockade of Na(+) currents after treatment with JMF2-1 (25.4 mM) was remarkably higher than that of lidocaine (0.18 mM), which is consistent with the weak anesthetic capacity of this analogue. In contrast, JMF2-1 was more potent than lidocaine in inhibiting allergen-induced histamine release and tracheal spasm. In in vivo settings methacholine-induced increase in lung resistance (145%) significantly reduced to 72% and 47% after lidocaine and JMF2-1 treatment, respectively. Both treatments inhibited by about 81% allergen-evoked eosinophil accumulation into the lung tissue.. Replacement of the 2,6-dimethyl radicals by the 2-trifluormethyl group on the benzene ring of lidocaine significantly reduces anesthetic activity, preserving its ability to prevent key aspects of the allergic inflammatory response in the lung.. Nebulized JMF2-1 might be a means of achieving the antiasthmatic effects of lidocaine without the anesthetic effects.

Topics: Anesthetics; Animals; Anti-Asthmatic Agents; Anti-Inflammatory Agents; Cell Line; Histamine; Leukocyte Count; Lidocaine; Lung; Mice; Ovalbumin; Rats; Rats, Wistar; Respiratory Hypersensitivity; Seizures; Sodium Channel Blockers; Sodium Channels; Trachea

The effect of methanolic extract of Asparagus pubescens was investigated on chemical, thermal-induced pain as well as fresh egg albumin-induced inflammation and pentylenetetrazol (PTZ)-induced convulsion in rodents. The extract dose-dependently (0.25-1.5 g/kg) inhibited acetic acid-induced writhing, formalin-induced pain licking and hot plate-induced pain in mice. The extract significantly inhibited both the fresh egg albumin-induced inflammation in rats as well as PTZ-induced convulsion in mice. These inhibitions were statistically significant (P < 0.02-0.001). It increased the latencies of both clonic and tonic convulsions and delayed their mortalities. Its ability to reduce both neurogenic and non-neurogenic pains may be related to its active constituents such as tannins, saponins, steroid and flavonoids.

Topics: Acetic Acid; Analgesics; Animals; Anti-Inflammatory Agents, Non-Steroidal; Anticonvulsants; Behavior, Animal; Formaldehyde; Hot Temperature; Inflammation; Liliaceae; Methanol; Mice; Nigeria; Ovalbumin; Pain Measurement; Pentylenetetrazole; Plant Extracts; Plant Roots; Rats; Seizures; Solvents

Topics: Anaphylaxis; Animals; Guinea Pigs; Isoproterenol; Male; Ovalbumin; Seizures

Topics: Aerosols; Anaphylaxis; Animals; Desensitization, Immunologic; Guinea Pigs; Injections, Intraperitoneal; Ovalbumin; Seizures; Time Factors

Topics: Anaphylaxis; Animals; Body Temperature Regulation; Guinea Pigs; Injections, Intraperitoneal; Injections, Intravenous; Movement Disorders; Ovalbumin; Seizures; Shivering