ovalbumin and Prostatitis

Topics: Animals; Chronic Disease; Chronic Pain; Disease Models, Animal; Drug Carriers; Enzyme-Linked Immunosorbent Assay; Immune Tolerance; Inflammation Mediators; Male; Mice; Mice, Inbred C57BL; Nanoparticles; Ovalbumin; Pelvic Pain; Peptides; Polylactic Acid-Polyglycolic Acid Copolymer; Prostatitis; Random Allocation

Current paradigms suggest that, despite the heterogeneity of myeloid-derived suppressor cells (MDSC), all Gr-1(+) CD11b(+) cells can exert suppressive function when exposed to inflammatory stimuli. In vitro evaluation shows that MDSC from multiple tissue sites have suppressive activity, and in vivo inhibition of MDSC enhances T-cell function; however, the relative capacity of MDSC present at localized inflammatory sites or in peripheral tissues to suppress T-cell responses in vivo has not been directly evaluated. In the current study, we observed that during a tissue-specific inflammatory response, MDSC inhibition of CD8(+) T-cell proliferation and IFN-γ production was restricted to the inflammatory site. Using a prostate-specific inflammatory model and a heterotopic prostate tumor model, we showed that MDSC from inflammatory sites or from tumor tissue possess immediate capacity to inhibit T-cell function, whereas those isolated from peripheral tissues (spleens and liver) were not suppressive without activation of iNOS by exposure to IFN-γ. These data suggest that MDSC are important regulators of immune responses in the prostate during acute inflammation and the chronic inflammatory setting of tumor growth, and that regulation of T-cell function by MDSC during a localized inflammatory response is restricted in vivo to the site of an ongoing immune response.

Topics: Adoptive Transfer; Animals; Arginase; CD11b Antigen; Cell Proliferation; Disease Models, Animal; Immune Tolerance; Inflammation; Male; Mice; Mice, Transgenic; Myeloid Cells; Nitric Oxide Synthase Type II; Ovalbumin; Phenotype; Prostatitis; Receptors, Chemokine; Recombinant Proteins; RNA, Messenger; T-Lymphocytes