ovalbumin has been researched along with Granuloma--Respiratory-Tract* in 2 studies
2 other study(ies) available for ovalbumin and Granuloma--Respiratory-Tract
Article | Year |
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Florid pulmonary inflammatory responses in mice vaccinated with Antigen-85 pulsed dendritic cells and challenged by aerosol with Mycobacterium tuberculosis.
Mice immunized by the intranasal route with dendritic cells harvested from the lungs and then pulsed with Ag85 (LDC-Ag85) were able to prime naive CD4(+) T cells in vivo. As a result splenic CD4(+) T cells from these immunized mice were able to produce IFNgamma following culture with Mycobacterium tuberculosis-infected antigen presenting cells. Hematoxylin and eosin stained lung sections from LDC-Ag85 immunized mice after they had been exposed to aerosol challenge with M. tuberculosis showed a florid infiltration of macrophages and lymphocytes into granulomas and parenchymal tissues when compared to lung sections from control groups implanted with dendritic cells pulsed with ovalbumin. In addition, using immunohistochemistry, these tissues appeared to have more CD4(+) and CD8(+) cells than the control groups. This was confirmed by flow cytometric analysis which showed that lung cell digests contained increased numbers of CD4 and CD8 interferongamma secreting cells. Despite this increase however, no evidence was seen that indicated that the LDC-Ag85 immunized mice were more resistant to M. tuberculosis infection than mice immunized with LDC pulsed with an irrelevant protein. Instead, the potent inflammatory response in the LDC-Ag85 resulted in serious consolidation of the lung tissue. Topics: Acyltransferases; Administration, Intranasal; Aerosols; Animals; Antigen Presentation; Antigens, Bacterial; Bacterial Proteins; Bacterial Vaccines; CD4-Positive T-Lymphocytes; Dendritic Cells; Female; Granuloma, Respiratory Tract; Immunization, Passive; Interferon-gamma; Mice; Mycobacterium tuberculosis; Ovalbumin; Pneumonia; Vaccination | 2002 |
Contribution of Th1 and Th2 cells to protection and pathology in experimental models of granulomatous lung disease.
Mice that had received adoptive transfer of DO11.10 TCR transgenic T cells polarized toward a Th1 or a Th2 phenotype were challenged with Ag-coated beads or with recombinant Mycobacterium tuberculosis expressing the OVA determinant. The resulting bead-induced pulmonary granulomas reflected the phenotype of the adoptively transferred T cells, with the Th2 cells promoting a fibrotic reaction. Mice receiving Th1 cells mounted an epitope-specific protective response to challenge with recombinant M. tuberculosis. Th2 recipients were characterized by enhanced weight loss and lung fibrosis during acute high-dose infection. The combination of TCR transgenic T cells and epitope-tagged mycobacteria provides a novel experimental model for investigation of the pathogenesis of tuberculosis. Topics: Adoptive Transfer; Animals; Antigens; Cells, Cultured; Cytokines; Disease Models, Animal; Female; Granuloma, Respiratory Tract; Injections, Intravenous; Lymphocyte Transfusion; Mice; Mice, Inbred BALB C; Mice, Transgenic; Microspheres; Mycobacterium tuberculosis; Ovalbumin; Spleen; Th1 Cells; Th2 Cells; Tuberculosis | 2001 |