ovalbumin and Foreign-Bodies

This study detected the expressions of microRNA-26a (miR-26a), miR-146a and miR-31 in lung tissues and BALF (bronchoalveolar lavage fluid) of asthma mice and children. Besides, cytokine levels of interleukin-5 (IL-5), IL-8, IL-12 and tumor necrosis factor-α (TNF-α) were detected as well. We aim to provide an experimental basis for clinical treatment of asthma.. Forty female BALB/c mice were randomly assigned into control group and asthma group, respectively. Mice in asthma group (n=20) were immunized by intraperitoneal injection of OVA (ovalbumin) and provoked by atomization inhalation of OVA from the 15th day for 10 days. Mice in control group (n=20) were immunized and provoked with isodose saline during the same period. At the 26th day, mice were sacrificed for collecting lung tissues and BALF. Besides, we enrolled 17 cases of asthma children and 13 cases of children with airway foreign body as controls. BALF of each subject was collected. Total cellular score and differential counting in BALF were recorded. Expression levels of miR-26a, miR-146a, and miR-31 were detected by reverse transcription-polymerase chain reaction (RT-PCR). Levels of IL-5, IL-8, IL-12, and TNF-α were detected by enzyme-linked immunosorbent assay (ELISA).. The total cellular score in BALF of asthma mice and asthma children was higher than that of controls (p<0.05). Percentages of eosinophils, neutrophils, and lymphocytes in BALF of asthma mice and asthma children were higher than those of controls, whereas the percentage of macrophages was lower (p<0.05). Levels of IL-5, IL-8, IL-12, and TNF-α in lung tissues of asthma mice were markedly elevated compared with those of controls (p<0.05). Similarly, levels of IL-5, IL-8, IL-12, and TNF-α were higher in BALF of asthma children than controls (p<0.05). RT-PCR data showed higher mRNA levels of miR-26a, miR-146a, and miR-31 in lung tissues of asthma mice than controls (p<0.05). The mRNA levels of miR-26a, miR-146a, and miR-31 in BALF of asthma children were highly expressed compared with those of controls as well (p<0.05).. MiR-26a, miR-146a, and miR-31 are involved in asthma progression mainly through regulating inflammatory factors and cells.

Topics: Adolescent; Animals; Asthma; Bronchoalveolar Lavage Fluid; Case-Control Studies; Disease Models, Animal; Disease Progression; Eosinophils; Female; Foreign Bodies; Humans; Inflammation Mediators; Lung; Lymphocytes; Male; Mice; MicroRNAs; Neutrophils; Ovalbumin; Up-Regulation; Young Adult

Antigen-specific CD8(+) cytotoxic T lymphocytes (CTLs) are key elements of immunological rejection in transplantation as well as cancer immunotherapy. Most tumors, however, are not immunologically rejected because they have self antigens, which are not recognized as the foreigner by CTLs. In this study, we hypothesized that "foreignizing" tumor cells by delivering non-self foreign antigens into the tumors would result in rejection by foreign antigen-reactive CTLs. As the model system to foreignize the tumors, we prepared a polymeric conjugate consisting of hyaluronic acid as the CD44(+) tumor-targeting ligand and ovalbumin (OVA) as a foreign antigen. When the conjugate was treated with CD44(high) TC-1 tumor cells, it was effectively taken up and allowed for displaying of antigenic OVA257-264 peptide at MHC class I on the surface of the cells. In addition, the conjugate was effectively accumulated into tumor tissue after its systemic administration to mice which are immunized with a vaccine for a vaccinia virus expressing OVA to generate OVA257-264 specific CTLs, resulting in substantial inhibition of tumor growth. Overall, these results suggest that the polymeric conjugates bearing foreign antigens may be innovative and promising cancer immunotherapeutic agents by foreignizing tumor cells, leading to immunological rejection.

Topics: Animals; Antigen Presentation; Apoptosis; Drug Delivery Systems; Female; Foreign Bodies; Genes, MHC Class I; Hyaluronan Receptors; Hyaluronic Acid; Immunotherapy; Mice; Mice, Inbred C57BL; Neoplasms, Experimental; Ovalbumin; Polymers; T-Lymphocytes, Cytotoxic; Tissue Distribution; Vaccinia virus

Guinea pigs with a genetically determined total deficiency of the fourth component of complement have been studied for various in vivo immunological functions. Passive cutaneous anaphylaxsis, contact and delayed hypersensitivity, and the cellular exudative response to a foreign body were normal. These animals also have normal direct and reverse passive Arthus reactions which suggest that they possess a mechanism to bypass C4 and directly activate late-acting complement components. This would appear to be an unequivocal demonstration of an alternate pathway in the complement sequence. Immune clearance of guinea pig erythrocytes sensitized with rabbit antibody was impaired in the deficient animals. Antibody production in C4-deficient animals was impaired for two of the three antigens studied.

Topics: Animals; Antibodies; Arthus Reaction; Complement System Proteins; Dinitrophenols; Erythrocytes; Female; Foreign Bodies; Guinea Pigs; Hypersensitivity, Delayed; Hypersensitivity, Immediate; Immunity; Immunologic Deficiency Syndromes; Male; Ovalbumin; Passive Cutaneous Anaphylaxis; Rabbits; Serum Albumin

Topics: Analgesics; Animals; Anti-Inflammatory Agents; Carrageenan; Croton Oil; Edema; Exudates and Transudates; Female; Foreign Bodies; Formaldehyde; Glycosides; Gossypium; Granuloma; Hydrocortisone; Ovalbumin; Phenylbutazone; Rats

Topics: Blood Cell Count; Blood Platelets; Bone Marrow Examination; Cortisone; Foreign Bodies; Immune Sera; Leukocyte Count; Megakaryocytes; Ovalbumin; Pharmacology; Rats; Research; Thrombocytosis; Thrombopoiesis