ovalbumin and Erythema

Lymphocyte stimulation tests (LST) were performed in five dogs sensitised with ovalbumin (OVA) and seven healthy dogs. In addition, all five OVA-sensitised and two control dogs were tested after two in vivo provocations with OVA-containing eye drops. The isolated cells were suspended in culture media containing OVA and were cultured for up to 12 days. Proliferation was measured as reduction in 5,6-carboxylfluorescein diacetate succinimidyl ester (CFSE) intensity by flow cytometry on days 0, 3, 6, 9 and 12. A cell proliferation index (CPI) for each day and the area under the curve (AUC) of the CPI was calculated for each dog. All OVA-sensitised dogs demonstrated increased erythema after conjunctival OVA application. The presence of OVA-specific lymphocytes was demonstrated in 2/5 OVA-sensitised dogs before and 4/5 after in vivo provocation. Using the AUC, the difference between OVA-sensitised and control dogs was significant in all three LST before in vivo provocation (P<0.05) and borderline significant (P=0.053) in 2/3 LST after provocation. The most significant difference in CPI was observed after 9 days of culture (P=0.001). This pilot study indicates that the LST allows detection of rare antigen specific memory T-cells in dogs previously sensitised to, but not concurrently undergoing challenge by a specific antigen.

Topics: Animals; Cell Division; Dog Diseases; Dogs; Erythema; Hypersensitivity; Lymphocyte Activation; Lymphocytes; Ovalbumin; Reference Values

Reports of allergy to bird meats are uncommon, and most have been in patients with "bird-egg syndrome.". We sought to evaluate 3 patients who reported allergic reactions to several avian meats, but who denied allergic reactions to eating eggs. The patients required yellow fever vaccine for entry into the military.. Patients were skin tested with commercial extracts of chicken, turkey, and egg, as well as with crude extracts made from dove and quail meat, and with yellow fever vaccine. Immunoblots for IgE antibody were performed by using the same materials used for skin testing plus extracts of duck and goose meat.. Skin tests were positive in all 3 patients to chicken, turkey, dove, quail, and yellow fever vaccine and negative to egg. This included some positive skin test responses to bird meats the patients denied ever having eaten. The vaccine was administered in graded doses. Immunoblots revealed IgE binding to several proteins of similar molecular weights in all of the avian meats but not to egg or yellow fever vaccine. Again, this included IgE antibody to some bird meats the patients denied ever having eaten.. Patients allergic to one bird meat may be allergic to others, including game birds, probably because of cross-reacting allergens. Such patients may have to exercise caution even when eating bird meats they have not previously ingested. The relationship of this allergy to yellow fever vaccine, if any, remains to be determined.

Topics: Adolescent; Adult; Allergens; Animals; Antibodies, Anti-Idiotypic; Blood Protein Electrophoresis; Cross Reactions; Erythema; Food Hypersensitivity; Geese; Humans; Hypersensitivity, Delayed; Immunoblotting; Immunoglobulin E; Male; Meat; Ovalbumin; Poultry; Quail; Skin Tests; Turkeys; Viral Vaccines; Yellow fever virus

Two non-sulfur containing ACE-inhibitors were tested concerning their local effect on experimental dermatitis in ovalbumin-sensitized guinea pigs. Enalaprilat but not cilazaprilat potentiated the ovalbumin-evoked inflammatory response. Furthermore, enalaprilat clearly enhanced the erythema evoked by substance P, whereas cilazaprilat did not. Concerning, the bradykinin-evoked erythema, enalaprilat significantly potentiated the response, whereas cilazaprilat only caused a slight increase. Our results suggest that different affinities for peptidases involved in degradation of inflammatory peptides can explain differences between the pro-inflammatory properties of enalaprilat and cilazaprilat.

Topics: Angiotensin-Converting Enzyme Inhibitors; Animals; Bradykinin; Cilazapril; Dermatitis; Drug Synergism; Enalaprilat; Erythema; Female; Guinea Pigs; Inflammation; Ovalbumin; Pyridazines; Substance P

Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Antitussive Agents; Carrageenan; Conjunctivitis; Cyclohexanes; Cyclohexenes; Edema; Erythema; Inflammation; Mice; Ovalbumin; Rats; Ultraviolet Rays

SCBH was induced by immunizing guinea pigs with a protein antigen and challenging 1 week later with a large intraperitoneal dose of the same antigen. Animals developed a delayed-onset, erythematous skin rash and dermal infiltration by basophils and eosinophils. The uveal tracts of these animals were infiltrated by eosinophils, as were several other internal organs. The eye is affected in SCBH, and as in other forms of ocular cell-mediated hypersensitivity, the eosinophil is a prominent cellular component of these reactions.

Topics: Animals; Basophils; Choroid; Ciliary Body; Eosinophils; Erythema; Female; gamma-Globulins; Guinea Pigs; Hypersensitivity, Delayed; Leukocyte Count; Male; Ovalbumin; Uveal Diseases

Delayed type skin reactions in guinea pigs have been assessed by measuring three parameters: increase in skin thickness, diameter of erythema and intensity of erythema. Some groups of animals were immunized with different protein antigens in Freund's complete adjuvant with or without cyclophosphamide (CY) pretreatment; others received a single high dose of antigen intravenously at the time of immunization. The results emphasize the importance of measuring all three parameters for several days after skin testing. The intensity or erythema was found to be an especially useful parameter for assessing CY-induced modification of delayed hypersensitivity (DH) reactions. The increase in skin thickness, which can be measured objectively, was also valuable as both immediate and DH reactions are characterized by induration. The diameter of erythema could only be measured accurately for a short time after skin testing. Furthermore, the effects of intravenous antigen and CY pretreatment were not reflected by that parameter.

Topics: Animals; Antigens; Arthus Reaction; Cyclophosphamide; Dinitrobenzenes; Erythema; Female; gamma-Globulins; Guinea Pigs; Hypersensitivity, Delayed; Intradermal Tests; Male; Ovalbumin; Serum Albumin, Bovine; Skin; Skinfold Thickness; Time Factors

Topics: Animals; Arthrodermataceae; Biopsy; Dogs; Epidermophyton; Erythema; Female; Humans; Injections, Intradermal; Male; Ovalbumin; Peptide Hydrolases; Plant Extracts; Pruritus; Skin; Tinea; Trichophyton; Tyrosine

Topics: Adrenalectomy; Animals; Anti-Inflammatory Agents; Arthritis; Calcium; Capillary Permeability; Carrageenan; Depression, Chemical; Dextrans; Edema; Erythema; Granuloma; Hemolysis; Hyaluronoglucosaminidase; Indicators and Reagents; Male; Malonates; Mice; Ovalbumin; Phenylbutazone; Phenylhydrazines; Prednisolone; Protein Denaturation; Radiation Injuries, Experimental; Rats; Serotonin; Ultraviolet Rays; Wound Healing

Topics: Animals; Binding Sites; Capillary Permeability; Chlorine; Coloring Agents; Dinitrophenols; Erythema; Female; Guinea Pigs; Hypersensitivity, Delayed; Hypersensitivity, Immediate; Injections, Intradermal; Injections, Intravenous; Injections, Subcutaneous; Nitrobenzenes; Ovalbumin; Serum Albumin, Radio-Iodinated; Time Factors